董晓莲,姚晴青,王学才,徐海涛,王晓丽,陈胜玉,唐智峰,郑英杰.母婴人群隐匿性乙型肝炎病毒感染及病毒株进化特征分析[J].Chinese journal of Epidemiology,2013,34(3):210-213 |
母婴人群隐匿性乙型肝炎病毒感染及病毒株进化特征分析 |
Prevalence of occult hepatitis B virus infection and its phylogenetic features among mother—teenager pairs |
Received:August 26, 2012 |
DOI: |
KeyWord: 隐匿性乙型肝炎病毒感染 显性乙型肝炎病毒感染 进化特征分析 基因型 |
English Key Word: Occult hepatitis B virus infection Overt hepatitis B virus Infection Phylogenetic analysis Genotype |
FundProject:浙江省医药卫生科学研究基金计划(A类)(2009A203);国家科技重大专项(2008ZXl0002—012) |
Author Name | Affiliation | E-mail | DONG Xiaolian | 313200 湖州, 浙江省德清县疾病预防控制中心 | | YAO Qingqing | Department of Epi&miology, School or Public Heahh, Fudun University. Ministry ofEducation the Key Laborator', PZic Health Safety | | WANG Xuecai | 313200 湖州, 浙江省德清县疾病预防控制中心 | | xu Haitao | 313200 湖州, 浙江省德清县疾病预防控制中心 | | WANG Xiaoli | 313200 湖州, 浙江省德清县疾病预防控制中心 | | CHEN Shengyu | 313200 湖州, 浙江省德清县疾病预防控制中心 | | TANG zhifeng | 313200 湖州, 浙江省德清县疾病预防控制中心 | | ZHENG Yingjie | Department of Epi&miology, School or Public Heahh, Fudun University. Ministry ofEducation the Key Laborator', PZic Health Safety | yjzheng@shmu.edu.cn |
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Abstract: |
目的了解母婴匹配人群隐匿性HBV感染(OBI)及显性和隐匿性病毒株s基因的变异。方法基础调查时随访135对母婴,填写调查表及采血样,采用ELISA检测HBsAg,Real—time PcR测定病毒载量,PCR扩增HBV S基因并进行进化分析。结果共采集母血94份,其子女血样101份,OBI感染率分别为10.0%和2.0%,母亲显著高于其子女;显性和隐匿性病毒株病毒载量中位数分别为399.9 IU/ml和247.6 IU/ml,两者差异无统计学意义;OBI病毒株有l株为B基因型、adw血清型,其余7株均为C基因型、adr血清型;显性HBV感染病毒株有15株为B基因型、adw血清型,其余8株为c基因型、adr血清型;OBI病毒株中C基因型比例显著高于显性HBV病毒株。结论母婴人群存在一定比例的OBI,应重视其预防。 |
English Abstract: |
Objective Prevalence of occult hepatitis B virus (HBV) infection (OBI) was investigated in a paired mother-teenager population and HBV S gene variation including overt and occult HBV,was determined.Methods A follow-up study based on an initial survey of 135 mother-teenager pairs was carried out through collection of questionnaires and blood samples HBsAg were detected by ELISA method,viral load by PCR amplification and HBV S gene by phylogenetic analysis.Results 102 pairs of subjects were followed-up.Blood samples from 94 mothers and 101children were collected.OBI prevalence in mothers was 10.0% (6/60),significantly higher than 2.0%(2/101) in teenagers.Medians of viral load were 399.9 IU/ml and 247.6 IU/ml in overt and occult HBV strains,but without significant difference.1 occult HBV strain belonged to genotype B with serotype adw while the other 7 were genotype C with serotype adr.15 of the overt HBV strains belonged to genotype B with serotype adw and the other 8 were genotype C with serotype adr.Proportions of genotype-C strains were significantly higher in occult HBV strains than in overt HBV strains.Conclusion OBI was seen in teenage-mother population. |
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