江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析

吴建茹; 朱一; 谢彦昕; 周良佳; 徐金水; 还锡萍; 王蓓

Abstract

吴建茹,朱一,谢彦昕,周良佳,徐金水,还锡萍,王蓓.江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析[J].Chinese journal of Epidemiology,2013,34(3):259-262

江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析

Analysis on Mycoplasma pirum infection in male HIVIAIDS patients and related 16S rRNA genes in Jiangsu province

Received:November 06, 2012

DOI：

KeyWord: 梨支原体 HIV感染者和艾滋病患者 pl基因 16S rRNA基因

English Key Word: Mycoplasma pirum HIV／AIDS p l gene 1 6S rRNA gene

FundProject:国家自然科学基金(30872156)

Author Name	Affiliation	E-mail
WU Jianru	Department of Epidemiology&Biostatistics, Schoof of Public Health, Southeast University, Nanjing 210009
ZHU Yi	Department of Epidemiology&Biostatistics, Schoof of Public Health, Southeast University, Nanjing 210009
XIE Yanxin	Department of Epidemiology&Biostatistics, Schoof of Public Health, Southeast University, Nanjing 210009
ZHOU Liangjia	Department of Epidemiology&Biostatistics, Schoof of Public Health, Southeast University, Nanjing 210009
XU Jinshui	Department of Epidemiology&Biostatistics, Schoof of Public Health, Southeast University, Nanjing 210009
HUAN Xiping	Jiangsu Provinciaf Centerfor Disease Control and Prevention
WANG Bei	Jiangsu Provinciaf Centerfor Disease Control and Prevention	wangbeilxb@Sell.edu.crl

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Abstract:

目的了解梨支原体(Mpi)在男性HIV感染者中的流行情况,复核鉴定部分Mpi 16S rRNA基因片段序列.方法以江苏省男性HIV/AIDS为研究对象,采集其首段尿,用巢式PCR法检测Mpi,并描述该人群中Mpi的感染情况.纯化PCR产物进行Mpi 16S rRNA基因片段序列测定,采用Mega 4.0软件的多序列排列程序对支原体临床株和下载序列进行同源位排列、比对,利用Neighbor-Joining构建系统发育树.将Mpi的pl基因序列信息输入Vector NTI Advance 11.0软件进行分析,并推算其编码的氨基酸序列,将推断出的理论氨基酸序列与其他支原体pl全序列进行同源性分析.结果 Mpi总感染率为21.5％.18株临床分离株几乎全部汇聚为一个分支,相似度＞90％,且与实验室保存Mpi标准株在同一分支,Mpi的pl蛋白与穿通支原体HF-2同源关系较近.结论 Mpi的感染率与以往调查结果相比明显增高,且临床分离株具有较高同源性.

English Abstract:

Objective To understand the incidence rates of both typhoid fever and paratyphoid fever in the high prevalent areas of Guizhou province so as to provide evidence for the development of programs on comprehensive intervention and effectiveness evaluation.Methods Six townships in Pingba county were selected as intervention areas while six townships in Kaiyang county were taken as control.All hospitals and clinics were classified into A,B and C types according to its level and the capacity of the blood culture.Surveillance on typhoid and paratyphoid fever was conducted based on all population and all hospitals,clinics and county CDCs among the patients with unknown fever.Results In the surveillance area in those two counties,there were 12 944 blood samples from patients with unknown fever which have been tested and cultured.Among them,200 strains of Salmonella including 16 typhoid strains,184 paratyphoid A strains were identified,with the total positive rate as 1.55％.The positive rate before the intervention program was higher than the after.The detection rate was 1.91％ in the type A hospitals.39 strains of Salmonella have been cultured from 2039 samples which accounting for 19.50％ (39/200) of the total strains.4315 blood samples were cultured at the 'Class B' sites which isolated 82 strains of Salmonella,accounting for 41.00％(82/200),with a detection rate as 1.90％.6590 samples were cultured at the 'Class C' sites,which identified 79 strains of Salmonella,accounting for 39.50％ (79/200),with a detection rate as 1.20％.The detection rate was much higher before the use of antibiotics than after using them (P＜0.05).The annual peak time of positive detection was in spring and fall.The outbreaks or epidemics often appeared in the same places,with farmers,students as the high-risk populations.Symptoms of both typhoid and paratyphoid fever were not typical.Conclusion Typhoid and paratyphoid monitoring programs which covered primary health care institutions in the high incidence area seemed to be effective in reflecting the pictures as well as the burden of both typhoid and paratyphoid.

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