| 付建光,艾静,靳淼,刘成,沙丹,姚萍,吴斌,祁贤,鲍昌俊,朱叶飞,汤奋扬.江苏省近期急性胃肠炎暴发中诺如病毒分子特征分析[J].Chinese journal of Epidemiology,2013,34(8):808-811 |
| 江苏省近期急性胃肠炎暴发中诺如病毒分子特征分析 |
| Molecular characteristics of acute gastrocenteritis outbreaks caused by norovirus, in Jiangsu province |
| Received:April 08, 2013 |
| DOI： |
| KeyWord: 诺如病毒|暴发|基因型|GⅡ.4/Sydney变异株 |
| English Key Word: Norovirus|Outbreak|Genotype|G Ⅱ.4-Sydney variant |
| FundProject:江苏省"十二五"科教兴卫工程(ZX201109);江苏省医学重点人才基金(RC2011084) |
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| Abstract: |
| 目的 了解江苏省胃肠炎暴发疫情中诺如病毒(NoV)感染状况,并对其病原分子流行病学特征进行初步研究.方法 收集江苏省2012年10月至2013年3月7起胃肠炎暴发疫情患者肛拭子标本共67份,采用实时荧光RT-PCR定性检测,NoV阳性标本用RT-PCR扩增其聚合酶区及VP1区基因片段进-步测序分型.结果 7起疫情均为NoV感染引起的暴发,检测阳性率为67.2％(45/67).全部45株阳性毒株序列分析表明7起疫情中除-起是由GⅡ.3型感染引起外,其余6起均由新GⅡ.4感染引起,其中38株毒株与2012年澳大利亚参考株GⅡ.4/Sydney株同源性均在99％以上.进-步对6株新型Sydney变异株的衣壳蛋白VP1区扩增测序,并与9株近年流行株的VP1区氨基酸序列比对,发现该6株毒株的VP1区部分氨基酸已出现突变,而氨基酸位点的突变是与病毒抗原性密切相关.结论 江苏省7起疫情暴发聚集且感染毒株型别单-,说明已出现新NoV变异株,其原型株GⅡ.4/Sydney已在国外多地引起暴发和流行,提示该毒株将是今后防控监测的重点. |
| English Abstract: |
| Objective To study both the epidemiologic and molecular characteristics of outbreaks caused by norovirus (NoV) with its variants,in Jiangsu.Methods 67 specimens from seven gastroenteritis outbreaks were collected from October 2012 to March 2013 in Jiangsu.NoV genegroup was detected by Real-Time RT-PCR.NoV portions of RdRp gene and VP1 gene were amplified under RT-PCR.Results Seven gastroenteritis outbreaks were caused by NoV.Among all the fecal specimens,45 (67.2％) showed positive to NoV G Ⅱ.Study on the genotype was conducted through analyzing the nucleotide sequence of RdRp gene.Based on the RdRp region,7 strains appeared to be G Ⅱ,with 3 and 38 strains belonged to G Ⅱ.4-Sydney variants.Results from phylogenetic analysis confirmed that 38 variants shared 99％ identity with G Ⅱ.4-Sydney.We also amplified the VP1 genes from 6 variants and comparing with 9 epidemic strains on the sequence amino acid sequence.All the strains showed mutation in amino acid sequence at some key sites which were closely related to the forming of neutralizing epitopes.Conclusion The short interval periods between all 7 NoV outbreaks with identical viral strain indicated the emergence of a new NoV variant in Jiangsu province,that had caused a number of epidemics abroad.Results from our study suggested that the development of monitoring programs on this novel G Ⅱ.4-Sydney variant should be a part of the NoV surveillance in Jiangsu province or even in the country. |
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