| 陈艳霞,马彩玲,陈志芳,张威.新疆维吾尔族和汉族宫颈癌患者微小RNA-143的表达及其临床病理意义[J].Chinese journal of Epidemiology,2013,34(11):1120-1124 |
| 新疆维吾尔族和汉族宫颈癌患者微小RNA-143的表达及其临床病理意义 |
| Clinical significance of miR-143 expression in women with cervical cancer of Uyghur and Hanethnicities,in Xinjiang CHEN |
| Received:April 27, 2013 |
| DOI：10.3760/cma.j.issn.0254-6450.2013.011.017 |
| KeyWord: 宫颈癌 微小RNA-143 临床特征 维吾尔族 |
| English Key Word: Cervical cancer MicroRNA一143 Clinicopathologic features Uygur |
| FundProject:国家自然科学基金(81160278，81101555)；新疆维吾尔自治区自然科学基金(2011211A070) |
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| Abstract: |
| 目的探讨微小RNA(miR)-143在新疆维吾尔(维)族和汉族宫颈癌患者中的表达及其与临床病理特征的关系。方法采用qRT-PCR方法检测正常宫颈及宫颈癌组织中miR-143的表达，分析miR-143在维、汉族间的差异及其与临床病理特征的关系。结果与正常宫颈组比较，miR-143在宫颈癌组中表达显著下降，差异有统计学意义(P<0.05)，但两民族人群间表达差异无统计学意义(P>0.05)。miR-143表达与淋巴结转移及肿瘤大小相关。有淋巴结转移的宫颈癌组，miR-143表达明显低于无淋巴结转移的宫颈癌组(Z=-2.127，P=0.033)；nO瘤直径>4 cm的宫颈癌组miR-143表达明显低于肿块直径≤4 cm的宫颈癌组(Z=-2.628，P=0.009)。ROC曲线下面积(AUC)分析显示，miR-143在不同肿块直径和淋巴结转移的宫颈癌组中，AUC分别为O.711和O.697，均>O.5。评价癌肿直径的敏感度和特异度分别为85.7%和62.2%；评价淋巴结转移的敏感度和特异度分别为72.2%和60.4%。miR-143表达与患者年龄、癌浸润深度、子宫旁浸润、FIGO分期、组织类型及病理分级无关。结论miR-143参与宫颈癌的发生发展，且无种族差异表达，可能成为宫颈癌诊断及病情预测的标记物。 |
| English Abstract: |
| Objective To explore the expression of microRNA(miR)-143 and relatedassociation to clinicopathologic features of cervical cancer in both Uygnr and Han women withcervical cancer in Xinjiang.Methods The expression levels of miR-143 in the specimens from 34non-tumor and 66 cervical cancer tissues.were examined by quantitative real-time PCR.Levels on theexpression of miR-143 in Xinjiang Uygur women and the correlations between the expression levelsof miR-143 and related clinicopathologic features of cervical cancer were analyzed.Results Theexpression 1evels of miR-l 43 were significantly lower in the tumor tissues than that in the non-tumortissues(P<0.05)but no significant difference was found between women with Uygur or Hanethnicities.Down.regulated miR-143 expression was associated with both the tumor size and lymphnode metastasis in patients with cervical cancer(Z=-2.628,P=0.009 and Z=-2.127,P=0.033respectively).No significant associations were found between the expression 1evels of miR-l43 andfactors as age,depth of tumor invasion,parametrial infiltration,clinical stage,types of histology andstage of differentiation.ROC curve analysis on miR-143 expression in cervical cancer patients withdifferent tumor sizes and lymph node metastasis：the rates on AUC were 0.7 1 l and 0.697,both largerthan 0.5.respectively.The sensitivity and specificity of evaluating tumor size were 85.7%and 62.2%.The sensitivity and specificity of lymph node metastasis were 72.2%and 60.4%.Conclusion Itseemed that miR-1 43 play an important role in the processes of generation and progression of cervicalcancer.However,there was no significant difference found between the different ethnic groups.Theexpression level of miR-143 might serve as a valuable adjuvant parameter for diagnosing andpredicting the state of cervical cancer. |
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