浙江省2008-2012年肠道病毒相关病毒性脑炎病原谱及分子流行病学特征分析

严菊英; 缪梓萍; 吕华坤; 周佳悦; 陈寅; 卢亦愚; 张严峻

Abstract

严菊英,缪梓萍,吕华坤,周佳悦,陈寅,卢亦愚,张严峻.浙江省2008-2012年肠道病毒相关病毒性脑炎病原谱及分子流行病学特征分析[J].Chinese journal of Epidemiology,2013,34(12):1189-1193

浙江省2008-2012年肠道病毒相关病毒性脑炎病原谱及分子流行病学特征分析

Etiological and molecular-epidemiological analysis on enterovirus associated encephalitis inZhejiang,2008-2012

Received:July 12, 2013

DOI：10.3760/cmaj.issn.0254-6450.2013.012.008

KeyWord: 肠道病毒相关脑炎病原谱 VPl基因

English Key Word: Enterovims associated encephalitis Etiological spectrum VPI gene

FundProject:国家科技重大专项(2012ZXl0004—210—002);浙江省科技厅项目(2012C33063)

Author Name	Affiliation	E-mail
Yan Juying	Department of Microbiology	jyyan1960@hotmail.com
Miao Ziping	Department of Infectious Disease, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051,China
Lv Huakun	Department of Infectious Disease, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051,China
Zhou Jiayue	Department of Microbiology
Chen Yin	Department of Microbiology
Lu Yiyu	Department of Microbiology
Zhang Yanjun	Department of Microbiology

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Abstract:

目的了解2008--2012年浙汀省肠道病毒相关病毒性脑炎病原谱及分子流行病学特征。方法从监测点采集疑似病毒性脑炎患者脑脊液和粪便样本利用RD和HeP-2细胞分离病毒,采用肠道病毒标准血清定型,并对分离株VPI基因测序,进行同源性与进化分析。结果从610例患者616份样本中分离到人类肠道病毒(HEY)127株(20.6%),其中柯萨奇病毒(CV)60株,埃可病毒(ECHO：E)67株,病毒血清型分别为CVA9、CVBl、CVB3～5、E3、E4、E6、E9、E14、E25、E30。2008～2012年优势株分别为CVB3、CVB5、E6、E30和E30。各分离株VPl基因序列全长834～918个核苷酸,与相应原型株核苷酸(nt)和氨基酸(aa)的同源性分别为76.7%～85.0%和91.1%。97.9%；浙江分离株型内差异最大为E6,nt和aa差异分别为20.4%和4.8%。基于VPl基因的进化分析结果表明。浙江分离株均位于HEV-B分支上,并显示出一定地域和时问效应；E6血清型内部又分成2小分支。结论2008-2012年浙江省病毒性脑炎的主要病原为HEV-B,涉及12个血清型；不同年份优势流行株不断变化,E30为绝对优势株；浙江E6分离株存在2个亚类流行株。

English Abstract:

0bjective In order to investigate etiology and molecular-epidemiologicalcharacteristics of enterovirus associated encephalitis(EAE)in Zhejiang，2008-2012.MethodCerebrospinal fluid and stopl specimens were collected from suspected EAE patients.who wereadmitted to our hospitals.RD and HeD-2 cell lines were used to isolate enterovirus(EV).Serotypesof these EV isolates were identified through neutralization test by using serotype specific anti-sera.VP l genes of these isolates were sequenced.compared and used for the construction of phylogenetictree.Results 127(20.6%)human enterovirus(HEY)strains were isolated from 61 6 samples，whichwere collected from 610 patients.Serotypes ofthese EV isolates，including 60 coxsackievims(CV)，and 67 Echovims(E)appeared to be CVA9，CVBl，CVB3-5，E3，E4，E6，E9，E14，E25 and E30，respectively.Predominant EV serotypes on EAE from 2008 to 2012 were seen as CVB3，CVB5.E6，E30 and E30.respectively.The full length of VPl genes from different EV isolates was between 834and 918 nucleotides.The VPl gene similarities between these isolates and the reference strains werefrom 76.7%to 85.0%(nucleotides 1evel)and 91.1%to 97.9%(amino acids 1evel).The VPl genesfrom E6 serotype isolates appeared most diverged，reaching 20.4%(nucleotides level)aad 4.8%(amino acids level).Based on the generated phylogenetic tree，all the EV isolates were fallen on thesame branch of HEV-B，and the isolates in the same serotype formed one sub-branch，suggesting thereexisted geographical and temporal effects.E6 isolates diverged into two branchlets。Conclusion EVsfrom HEV-B were the etiologic agents for EAE in Zhejiang province from 2008 to 201 2.All these EVisolates showed 1 2 serotypes，with predominant isolates varied every year.E30 was determined as themost dominant serotype while serotype E6 diverged into two sub—genetypes.

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