| 买欢,高燕,潘孝本,韩进超,丛旭,魏来.中国7个地区诺如病毒G II．4／Sydney 2012变异株分析[J].Chinese journal of Epidemiology,2014,35(2):157-162 |
| 中国7个地区诺如病毒G II．4／Sydney 2012变异株分析 |
| Study on norovirus G II .4/Sydney 2012 variant in China |
| Received:August 19, 2013 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.02.012 |
| KeyWord: 诺如病毒 GⅡ．4基因型 变异株 衣壳蛋白基因 |
| English Key Word: Norovirus G II .4 genotype Variant Capsid gene |
| FundProject: |
| Author Name | Affiliation | E-mail | | Mai Huan | Institute of Hepatology,People's Hospital, Peking University, Beijing 100044, China | | | Gao Yan | Institute of Hepatology,People's Hospital, Peking University, Beijing 100044, China | Email:gaoyan6384@ 163.com | | Pan Xiaoben | 肝病研究所 | | | Han Jinchao | 肝病研究所 | | | Cong Xu | 肝病研究所 | | | Wei Lai | Institute of Hepatology,People's Hospital, Peking University, Beijing 100044, China | |
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| Abstract: |
| 目的 分析2012--2013年冬季中国地区诺如病毒新流行株GII．4／Sydney 2012的衣壳蛋白区核苷酸与氨基酸变异特点。方法 对已获得的22份2012--2013年冬季北京地区GⅡ．4／Sydney2012株进行全衣壳蛋白区基因扩增。从GenBank检索中国其他地区的GⅡ．4／Sydney 2012株衣壳蛋白区核苷酸序列。对获得的GII．4／Sydney 2012株的衣壳蛋白区核苷酸及氨基酸序列与往年GⅡ．4流行株进行系谱分析。结果 获得中国7个地区（北京、上海、江苏、湖州、荆州、香港和台湾）的GⅡ．4／Sydney 2012株资料共38份（至少包含完整VPl核苷酸序列）。GH．4／Sydney 2012株之间VPl核苷酸差异为0．1％～3．3％，氨基酸差异为0～3．1％。系谱分析发现GⅡ．4／Sydney2012株与Apeldoorn 2008和NewOrleans 2009起自共同的分支。中国和悉尼GH．4／Sydney 2012代表株的A、D、E抗原表位氨基酸序列组合有两种：TSRN-GTT-SNT和TSRN-STT-SNT。结论 G II．4／Sydney 2012株已在中国多个地区出现，各地区病毒株同源性较高。G H．4／Sydney 2012株的抗原表位氨基酸组合发生明显改变。 |
| English Abstract: |
| Objective To study the nucleotide and amino acid sequences of norovirus G II .4/Sydney 2012 variants, in China. MethodsTwenty-two stool specimens, confirmed as G II .4/Sydney 2012- positive were collected from Beijing in the winter of 2012-2013. RT PCR was performed to target the complete capsid gene. G II .4/Sydney 2012 strains from other regions in China were searched and obtained from the GenBank. Nucleotide and amino acid sequences of G II .4/Sydney 2012 strains were analyzed, using the CLUSTAL X ( Version 1.83 ) and followed by phylogenetic analysis using Mega version 5.1. Results The complete major capsid nucleotide sequences of thirty-eight G II .4/ Sydney 2012 strains from seven regions in China were obtained. The VP1 nucleotide and amino acid sequences diversity were 0.1%-3.3% and 0-3.1%，respectively. Result from phylogenetic analysis demonstrated that the G II .4/Sydney 2012 variant shared a common ancestor with both the dominant norovirus G Ii .4 variants Apeldoorn 2008 and the New Orleans 2009. G II .4lSydney 2012 variants
appeared to have had two A/D/E site combinations at the amino acid level, TSRN-GTT-SNT and TSRN-STT SNT. Conclusion G II .4/Sydney 2012 variant had been circulating in many regions in China. There seemed a high nucleotide and amino acid identity among G II .4/Sydney 2012 strains collected from China. G II .4/Sydney 2012 variants showed different A1DlE site combination from other pandemic G II .4 variants. |
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