| 石丽媛,叶蕊,董珊珊,郭英,杨光璨,张蓉,崔志刚,李伟,王鹏.云南省鼠疫菌Fse I酶切分型及其流行病学意义[J].Chinese journal of Epidemiology,2014,35(2):182-185 |
| 云南省鼠疫菌Fse I酶切分型及其流行病学意义 |
| Genotyping and its epidemiological significance on Yunnan Yersinia pesos under FseI enzyme digestion method |
| Received:October 18, 2013 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.02.018 |
| KeyWord: 鼠疫耶尔森菌 脉冲场凝胶电泳 Fse I酶切方法 |
| English Key Word: Yersinia pesos Pulse field gel electrophoresis Fse I enzyme digestion method |
| FundProject:国家自然科学基金(81160354)；卫生部行业基金(201202021)；云南省应用基础研究(2011FB134) |
| Author Name | Affiliation | E-mail | | Shi Liyuan | Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China | | | Ye Rui | School of Public Health, Dali University, Dali, Yunnan Province | | | Doag Shanshan | Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China | | | Guo Ying | Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China | | | Yang Cuangcan | Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China | | | Zhang Rong | School of Public Health, Dali University, Dali, Yunnan Province | | | Cui Zhigang | 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室 | | | Li Wei | 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室 | | | Wang Peng | Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China
School of Public Health, Dali University, Dali, Yunnan Province | Email:wp030801@126.com |
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| Abstract: |
| 目的 构建云南省鼠疫菌Fse I酶切的脉冲场凝胶电泳（PFGE）图谱库并探讨其流行病学意义。方法 采用限制性内切酶Fse I对云南家、野鼠型及丽江玉龙鼠疫菌株进行酶切分析，并聚类分析电泳图谱。结果 30株被试菌株分为16种PFGE型别，其相似性系数为79．8％～100．0％；16种PFGE基因型可以分为4个簇，除EV76自成一簇，其余3个簇分别为家鼠型基因簇、野鼠型基因簇及玉龙基因簇。结论 云南省鼠疫菌PFOE基因型与生态型、生物型相吻合，具有一定的区域聚集性，而丽江玉龙鼠疫菌为一个独立基因簇。 |
| English Abstract: |
| Objective To create the fingerprint library of Yunnan Yersinia pesos by pulse field gel electrophoresis ( PFGE ) with Fse I enzyme digestion method and to study its epidemiological significance.Methods We used rare cutting restriction enzyme Fse I to digest Yunnan Yersinia pesos strains that were isolated from foci including Rattus flavipectus Plague Focus, Apodemus chevrieri- Eothenomys miletus Plaguc Focus and Yulong Plague Focus. Fingerprints to Bionumerics software were used for cluster analysis.Results 30 tested strains were divided into 16 genotypes with the similarity value as 79.8%一100.0%.16 genotypes wee formed into 4 clusters, with one consisted of only EV76 while the other three belonged to Rattus flavipectus,Apodemus chevrieri- Eothenomys miletus and Yulong clusters, respectively. Conclusion PFGE genotypes of Yunnan Yersinia pesos accorded with its ecotypes and biovars, with clustered regional features. The strains isolated from Yulong showed an unique PFGE type, indicating a new clone group was identified. |
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