| 李剑军,张鸿满,沈智勇,周月姣,方宁烨,王斌,王江伟,唐振柱.集合核酸检测用于低档场所女性性工作者HIV急性感染诊断及发病率估计[J].Chinese journal of Epidemiology,2014,35(3):259-261 |
| 集合核酸检测用于低档场所女性性工作者HIV急性感染诊断及发病率估计 |
| The role of RNA pooling technique Jin the diagnosis of acute HIV infection and tIle estimation Oil HIV incidence among low-grade-venues female sex workers |
| Received:September 27, 2013 Revised:June 23, 2012 |
| DOI： |
| KeyWord: HIV急性感染 集合核酸检测 女性性工作者 发病率 |
| English Key Word: Acute HIV infection RNA pooling Female sex workers Incidence |
| FundProject:广西科学研究与技术开发计划项目(桂科攻1298003-1--4) |
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| Abstract: |
| 目的评价集合核酸检测方法在低档场所女性性工作者(FSWs)HIV急性感染诊断中的应用价值。方法对广西壮族自治区2011年低档场所FSWs进行调查，并采集血液，采用胶体硒法对HIV抗体初筛检测；对初筛阴性者采用集合核酸检测方法检测HIVRNA，若为阳性，3个月内随访并采集血液进行确证；应用估算公式计算HIV年发病率。结果共收集6 469例FSWs的血液样本，HIV抗体初筛阳性139洌，阳性率为2.15%。x,J 6 330例初筛阴性者进行集合核酸检测，7例为HIVRNA阳性。3个月内随访检测，6例出现HI、Ll抗体阳转。诊断为HIV急性感染者i 2011年广西低档场所FSWs的EIIV年发病率为1.45(95%CI：1.17～1.76)／100人年。结论集合核酸检测用于HIV传播的高危人群检测可以发现急性感染者。 |
| English Abstract: |
| ObjectiveTo examine the feasibility of RNA pooling technique in the diagnosis Off acute HIV infection among female sex workers(FSWs)working at the lOW.grade venues.Methods Plasma samples from the lOW-grade-venue FSWs in Guangxi.in 20 1 1 were tested for HIVantibody using the rapid testing meth,x1.All samples which were HIV antibody negative in the rapidtesting were tested for HIV RNA with RNA pooling technique.FSWs who showed HIV RNA positivewere tested for HIV antibody by、7i7estern blot method in 3 months.The HIV incidence in thelow-grade venue FSWs was counted under the estimation formula.ResuiIts There were 6 469 cases of FSWs who were recruited in this study.ThrOUgh rapid testing.Results showed that HIV antibodvwas positive in 1 39 cases.with the positive rate as 2.1 5%.6 330 FSWs with HlV antibody negativewere tested by HIV RNA pooling method，with 7 of them showing HIV RNA positive.in which 6 cases showed HIv-1 antibody seroconversion.thus were diagnosed as acute HIV infection.HIV incidence in low.grade FSWs appeared to be 1.45 per 100 person years(95%CI：1.17-1.76 per 100 person years)in Guangxi.Conelusio.il Other than regular routine HIV antibody testing.it seemed necessary to adopt the HIV RNA pooling strategy in high-risk groups such as FSWs.SO as tO early detect the HIV infection and to timely perforill the intervention or treatment programs to prevent sexual transmission of HI v |
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