| 费满冬,李佳圆,杜旌畅,游嘉,张韶凯,何微,康乐妮,赵方辉,乔友林,司玉芝,樊小平,陈汶.子宫颈癌高危妇女中人乳头瘤病毒16和18型血清抗体分布特征[J].Chinese journal of Epidemiology,2014,35(5):514-518 |
| 子宫颈癌高危妇女中人乳头瘤病毒16和18型血清抗体分布特征 |
| Distribution of serum antibodies against human papillomavirus 16 and 18 among high-risk women to cervical cancer |
| Received:September 29, 2013 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.05.010 |
| KeyWord: 人乳头瘤病毒 基因型别 血清抗体 酶联免疫吸附试验 |
| English Key Word: Human papillomavirus Genotypes Serum antibody Enzyme-linked immunosorbent assay |
| FundProject:国家高技术研究发展计划(863计划)(2012AA02A408) |
| Author Name | Affiliation | E-mail | | Fei Mandong | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Department of Epidemiology, West China School of Public Health, Sichuan University | | | Li Jiayuan | Department of Epidemiology, West China School of Public Health, Sichuan University | | | Du Jingchang | Institute of Labor and Environmental Hygiene, Chongqing Medical University | | | You Jia | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | Zhang Shaokai | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | HeWei | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | Kang Leni | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | Zhao Fanghui | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | Qiao Youlin | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | | | Si Yuzhi | Women and Children Hospital in Xinmi City of Henan Province | | | Fan Xiaoping | Women and Children Hospital in Xinmi City of Henan Province | | | Chen Wen | Cancer Instituteand Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China | chenwen@cicams.ac.cn |
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| Abstract: |
| 目的 分析HPV16和18型 （HPV16/18） 血清抗体在高危人群中的分布。 方法 召回952名参加2011年河南省新密市子宫颈癌筛查阳性妇女，于2012年采集子宫颈口脱落细胞检测HPV DNA，采用ELISA检测血清HPV16/18抗体。结果 952名妇女中230例 （24.2%） HPVDNA阳性，HPV16/18 病毒样颗粒 （VLP） 抗体阳性率分别为23.2%和6.5%，任意一种HPV16/18VLP 抗体阳性率为 26.8%。HPV16/18 抗体 GMT 分别为 79.1 YU（Yangshengtang Unit）/ml和125.0 YU/ml。HPV16抗体阳性率在不同年龄、 不同病毒载量和不同子宫颈病变组的差异有统计学意义 （P<0.05）； HPV18抗体阳性率与病毒载量相关 （P<0.01），且在不同病理等级中其滴度分布不同，差异有统计学意义 （P<0.01）。结合两年HPV DNA检测结果，HPV持续感染的妇女HPV16/18抗体阳性率高于HPV新发感染和无HPV感染妇女，差异有统计学意义 （P<0.001）； 相对于无HPV感染妇女，HPV一过性感染者HPV16抗体阳性率和滴度均较高，差异有统计学意义（P<0.001）。结论 HPV16/18 抗体在调查的高危妇女中阳性率较高，且与年龄、 病毒载量、 子宫颈病变程度以及既往感染史有关，病毒载量高、 病变程度重或有既往感染史者更易产生抗体。 |
| English Abstract: |
| Objective To explore the distribution of serum antibodies against human papillomavirus(HPV)16/18 among women at high-risk for cervical cancer. Methods All women when tested positive for anyone of the cervical cancer screening programs, from Xinmi county of Henan province in 2011, were recruited as the subjects of this study. Cervical exfoliated cells were collected, using cervical brush for HPV DNA testing, and 10 ml venous blood was drawn for HPV-16, 18 serum antibodies testing，by enzyme-linked immunosorbent assay. Results Among the 952 women unders study, 230 cases(24.2%) showed HPV DNA positive, with positivity rates of HPV16 and 18 L1 virus-like particle(VLP) antibodies as 23.2% and 6.5%, respectively. The overall positivity rate of any type of HPV16, 18 VLP antibodies was 26.8%. Geometric means of HPV16, 18 VLP antibody titers were 79.1(Yangshengtang Unit, YU/ml) and 125.0(YU/ml). Positivity rate of HPV16 antibody was significantly associated with age, viral load of HPV DNA, and cervical lesion severity(P<0.05). Seropositvity of HPV18 was also increasing with the increase of viral load(P<0.01) with different cervical lesion significantly showing different titer of HPV18 antibody(P<0.01). Based on the results of HPV DNA detection among the two years of study，women with HPV persistent infection showed significant higher positive rate of HPV16/18 antibodies than women who did not have HPV infection or emerging infection(P<0.001). When comparing to those women without HPV infection， the ones with transient infection showed higher seropositivity rates on both HPV16 antibodies and titer of HPV16 antibody(P<0.001). Conclusion Seroprevalence rates on HPV16 and 18 among the unvaccinated high-risk women in Henan were high. Prevalence of both HPV16 and 18 antibodies were correlated with age, viral load, cervical lesion and history of infection. Women with high viral load, high grade cervical lesion or history of infection would more likely to be seropositive. |
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