| 宫连凤,姜梅,刘娟,韩文清,刘靖宇,孙振璐,王志昱,高巧,邢玉芳,丁淑军,王显军.山东省烟台市人与动物新型布尼亚病毒感染调查及同源性分析[J].Chinese journal of Epidemiology,2014,35(5):524-527 |
| 山东省烟台市人与动物新型布尼亚病毒感染调查及同源性分析 |
| Prevalence and homology analysis on human and animals severe fever with thrombocytopeniasyndrome virus infection in Yantai of Shandong province |
| Received:November 27, 2013 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.05.012 |
| KeyWord: 发热伴血小板减少综合征布尼亚病毒 动物 人群 感染 同源性 |
| English Key Word: Severe fever with thrombocytopenia syndrome bunyavirus Animal Human Infection Homology |
| FundProject: |
| Author Name | Affiliation | E-mail | | Gong Lianfeng | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Jiang Mei | Yantai Center for Disease Control and Prevention, Yantai 264003, China | ytjiangmei@163.com | | Liu Juan | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Han Wenqing | Yantai Center for Disease Control and Prevention, Yantai 264003, China | ythwq405@aliyun.com | | Liu Jingyu | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Sun Zhenlu | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Wang Zhiyu | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Gao Qiao | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Xing Yufang | Yantai Center for Disease Control and Prevention, Yantai 264003, China | | | Ding Shujun | Institute for Viral Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention | | | Wang Xianjun | Institute for Viral Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention | |
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| Abstract: |
| 目的 了解山东省烟台市人和动物发热伴血小板减少综合征布尼亚病毒 （SFTSV）感染及流行情况。方法 2011年4－11月分别在烟台市的蓬莱和莱州2个SFTS病例高发地区连续采集与人密切接触的5种家养动物 （羊、 牛、 猪、 犬、 鸡） 血清标本3 576份，应用双抗原夹心ELISA方法和Real time RT-PCR方法检测血清中SFTSV总抗体和病毒核酸； 观察不同月份感染情况； 采集两地人群血清2 590份，应用间接ELISA方法检测SFTSV IgG抗体； 用Vero细胞从核酸阳性的人和动物血清中分离病毒，通过RT-PCR方法对 SFTSV S片段进行序列扩增、 同源性分析。结果 3 576份动物血清标本中SFTSV血清总抗体阳性1 439份，阳性率为40.24%，病毒核酸阳性163份，阳性率为4.56%。其中羊、 牛、 鸡、 犬、 猪抗体阳性率分别为62.78%、 52.97%、 45.56%、28.73%和1.45%，核酸阳性率分别为5.72%、 4.63%、 3.02%、 5.25%和3.73%。动物体内的抗原抗体随季节消长而变化。2 590份人群血清SFTSV IgG抗体阳性率为5.41%。对10株来自人的毒株和3株来自动物的毒株进行S片段基因序列扩增分析，显示其同源性在95.23%～100.00%，与国内其他省市分离毒株比较，其同源性在94.72%～99.13%，高度同源。结论 烟台地区存在SFTSV流行，人与家养动物普遍易感，其基因序列高度同源，提示家养动物可能作为SFTSV的增殖宿主和扩散宿主，应引起高度重视。 |
| English Abstract: |
| Objective To learn the prevalence of infection of human and animals severe fever with thrombocytopenia syndrome bunyavirus (SFTSV)in Yantai, Shandong province, and to analyze the pathogenic features of SFTSV as well as its relationship between human and animal hosts. Methods From April to November in 2011, 3 576 serum samples were collected from domesticated animals, including sheep, cattle, pigs, dogs, chickens, in Laizhou and Penglai areas where fever with thrombocytopenia syndrome frequently occured among local residents. Total SFTSV antibodies and virus-specific nucleic acids of the serum were tested by ELISA and Real time RT-PCR, respectively. SFTSV infection on each animal was observed in different months. 2 590 human serum samples were also collected in Laizhou and Penglai areas, with IgG antibodies tested by ELISA. Virus was isolated with Vero cells from the serum which SFTSV viral nucleic acids were positive. S fragments were amplified by RT-PCR and sequenced, with homology analysis conducted on these sequences. Results The overall positive rate of serum samples from animals on the total SFTSV antibodies was 40.24%(1 439/3 576) while the positive rate for specific nucleic acids was 4.56%(163/3 576). The positive rates for SFTSV antibodies were 62.78%, 52.97%, 45.56%, 28.73%, 1.45% and the positive rates for specific nucleic acids were 5.72%, 4.63%, 3.02%, 5.25% and 3.73%, in sheep, cattle, chickens, dogs, pigs. respectively. The antigens/antibodies for SFTSV in animals changed seasonally. The overall positive rate for SFTSV IgG antibody from 2 590 human samples was 5.41%. Thirteen virus strains were isolated from these serum samples(10 strains from human and 3 strains from animals). The nucleotide homology of 13S fragments' sequences ranged from 95.23% to 100.00% and the nucleotide homology with the isolates from other provinces were between 94.72% and 99.13%. The homology was considered to be high. Conclusion High prevalence of SFTSV infections occured both in human and domestic animals in Yantai city. The nucleotide sequences of SFTSV were highly homologous among human and domestic animals. The findings suggested that domesticated animals might serve as SFTSV proliferation and the hosts for transmission thus should be attached great importance. |
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