| 赵璇,张力,李杰,阚飙,梁未丽.中国O1群El Tor霍乱弧菌产毒株表型多态性研究[J].Chinese journal of Epidemiology,2014,35(5):573-575 |
| 中国O1群El Tor霍乱弧菌产毒株表型多态性研究 |
| Phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains identified in China |
| Received:February 10, 2014 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.05.023 |
| KeyWord: 霍乱弧菌 生物型 ctxB rstR |
| English Key Word: Vibrio cholerae Biotype ctxB rstR |
| FundProject:国家自然科学基金（81171640） |
| Author Name | Affiliation | E-mail | | Zhao Xuan | State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Tianjin Centre for Disease Control and Prevention | | | Zhang Li | State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Li Jie | State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Kan Biao | State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Liang Weili | State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | liangweili@icdc.cn |
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| Abstract: |
| 目的 了解近50年中国不同地区分离的O1群El Tor型霍乱弧菌产毒株的生物表型特征变化。方法 采用多粘菌素B敏感试验、第Ⅳ组霍乱噬菌体裂解试验、VP试验和溶血实验进行表型特征分析。结果 生物型表型特征分析表明133株菌具有典型的El Tor生物型表型特征，其余251株菌呈现不典型的El Tor生物型表型特征；综合ctxB、rstR基因型和生物型表型特征分析，385 株检测菌株中 64 株为典型 El Tor 生物型菌株，21 株菌有典型的 El Tor 生物型表型特征但携带古典型ctxB基因，杂合型特征菌有280株，根据ctxB、rstR和表型特征的不同组合可再分为45个杂合型。结论 中国O1群El Tor霍乱弧菌菌株呈现明显的表型多态性，传统的表型分型特征不能有效区分古典生物型和El Tor生物型菌株。 |
| English Abstract: |
| Objective To understand the phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains isolated from different provinces in China during the last 50 years. Methods Traditional biotyping testings including susceptibility to polymyxin B，sensitivity to group Ⅳ phage， Voges-Proskauer test and haemolysis of sheep erythrocytes were conducted. Results Data from Biotype-specific phenotype analysis revealed that only 133 isolates carryed the typical El Tor phenotypes while the other 251 isolates displayed atypical El Tor phenotypes. Combined with ctxB， rstR genotypes and phenotypic characteristics，64 isolates were identified as typical El Tor biotype，21 were El Tor variants that showing the typical El Tor biotype-specific phenotype but with ctxB class. 280 isolates were defined as the hybrid groups with traits of both classical and El Tor biotypes that could be further classified into 45 groups，based on the combination of genotypes of ctxB，rstR and phenotypic characteristics. Conclusion Toxigenic Vibrio cholerae O1 El Tor strains that isolated from different provinces in China displayed high phenotypic diversity. The traditional biotype traits could not be used to correctly distinguish the two different biotypes. |
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