福建省HIV-1 新近感染者流行毒株基因亚型及其流行特征分析

邓永岳; 张春阳; 严延生; 颜苹苹; 吴守丽

Abstract

邓永岳,张春阳,严延生,颜苹苹,吴守丽.福建省HIV-1 新近感染者流行毒株基因亚型及其流行特征分析[J].Chinese journal of Epidemiology,2014,35(6):714-719

福建省HIV-1 新近感染者流行毒株基因亚型及其流行特征分析

Genetic subtype and epidemiological feature of HIV-1 circulating strains among recently infected patients in Fujian province

Received:February 12, 2014

DOI：

KeyWord: 艾滋病病毒新近感染者亚型

English Key Word: HIV-1 New recent infections Subtype

FundProject:福建省卫生厅青年科研基金（2012-1-12）

Author Name	Affiliation	E-mail
Deng Yongyue	Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, China
Zhang Chunyang	Fujian Provincial Center for Disease Control and Prevention
Yan Yansheng	Fujian Provincial Center for Disease Control and Prevention	yysh@fjcdc.com.cn
Yan Pingping	Fujian Provincial Center for Disease Control and Prevention
Wu Shouli	Fujian Provincial Center for Disease Control and Prevention

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Abstract:

目的了解福建省HIV-1 新近感染者流行毒株的亚型分布和流行特征。方法随机抽取福建省2011－2012 年使用BED捕获酶免疫试验检出的104 例新近感染者血样标本，采用反转录巢式PCR扩增,gag、pol 和env 基因片段，并测定和分析核酸序列。结果目标人群中存在B亚型和01_AE、07_BC、08_BC、55_01B 四种流行重组型，其中以01_AE 和07_BC 流行重组型为主，分别占45.68%和35.80%。基因亚型的流行特征分析显示，亚型分布中01_AE 所占比例下降，07_BC 所占比例上升;性传播是主要感染途径，其中同性传播比例明显上升。结论福建省HIV-1 流行毒株亚型分布众多，随着时间推移，亚型构成和传播途径已发生改变，应加强对HIV-1感染的分子流行病学趋势动态监测。

English Abstract:

Objective In order to evaluate the distribution of genetic subtypes and epidemiological feature of HIV-1 circulating strains in Fujian province. Methods Blood samples and epidemiological data were collected from 104 newly infected patients who were distinguished by BED-CEIA methodology，during 2011-2012. Viral sequences（n＝81）of HIV-1 gag，env，and pol segments were amplified by nested PCR. Results Subtypes B and four Circulating Recombinant Forms，（CRF01_AE，CRF07_BC，CRF08_BC and CRF55_01B） were found in the samples， CRF01_AE（45.68%）and CRF07_BC（35.80%）were the two main HIV-1 strains in Fujian province. Compared with previous data，the proportion of CRF07_BC rose significantly while it gradually decreased in CRF01_AE. Heterosexual contact was still the principal transmission route in Fujian province，but the number of infection among men-who-have-sex-with- men grew rapidly. Conclusion Results from this study suggested that different subtypes of HIV-1 strain existed in Fujian province. The distribution of subtypes and the mode of transmission were changing with the progress of epidemic. Dynamic monitoring of the molecular epidemiology trends of HIV-1 infection should be enhanced.

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