云南省德宏州2012年HIV-1耐药传播警戒线调查

陈敏; 王继宝; 邢辉; 马艳玲; 姚仕堂; 陈会超; 杨锦; 李艳玲; 段松; 贾曼红

Abstract

陈敏,王继宝,邢辉,马艳玲,姚仕堂,陈会超,杨锦,李艳玲,段松,贾曼红.云南省德宏州2012年HIV-1耐药传播警戒线调查[J].Chinese journal of Epidemiology,2014,35(8):909-912

云南省德宏州2012年HIV-1耐药传播警戒线调查

Study on HIV-1 related genetics and threshold on drug resistance in Dehong prefecture of Yunnan province in 2012

Received:February 26, 2014

DOI：10.3760/cma.j.issn.0254-6450.2014.08.008

KeyWord: 人类免疫缺陷病毒基因型耐药警戒线调查抗病毒治疗

English Key Word: Human immunodeficiency virus Genotype Drug resistance threshold survey Antiretroviral therapy

FundProject:“十二五”国家科技重大专项（2012ZX10001-002）

Author Name	Affiliation	E-mail
Chen Min	Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Wang Jibao	Dehong Center for Disease Control and Prevention
Xing Hui	National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention
Ma Yanling	Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Yao Shitang	Dehong Center for Disease Control and Prevention
Chen Huichao	Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Yang Jin	Dehong Center for Disease Control and Prevention
Li Yanling	Dehong Center for Disease Control and Prevention
Duan Song	Dehong Center for Disease Control and Prevention
Jia Manhong	Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China	dhduansong@sina.com

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Abstract:

目的调查2012年云南省艾滋病高流行区德宏州未经抗病毒治疗的HIV感染者的基因型分布和耐药株传播水平。方法根据HIV耐药警戒线调查实施方案，对2012年1-8月德宏州符合要求的60份年龄在16～25岁新发现的HIV-1感染者的血浆样本进行HIV-1耐药基因型检测和耐药株传播水平分析。结果 52份样本完成基因型及耐药鉴定，其中中国籍占59.6%，缅甸籍占40.4%。通过进化分析对pol区进行分型，38.5%的样本为独特型重组（URF），其他依次为C 亚型（34.6%）、CRF01_AE（21.2%）、CRF08_BC（3.8%）和B亚型（1.9%）。在2个序列中各检测到1个针对非核苷类反转录酶抑制剂（NNRTI）类药物的耐药突变位点，按照耐药警戒线的统计方法估算耐药株流行率为5%～15%，属于中度水平。结论德宏州新感染人群中存在复杂的HIV-1基因型，HIV-1耐药株已处于中度流行水平。

English Abstract:

Objective To study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2012. Methods Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS),60 plasma samples of recently reported HIV-infected individuals between 16 and 25 years old were collected in Dehong prefecture from January to August 2012. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype and the prevalent levels of HIV-1 drug resistance transmission were analyzed. Results 52 plasma samples were successfully sequenced and analyzed. Among them,59.6% were Chinese,and the rest (40.4%) were Burmese. Based on pol sequences,identified HIV genotypes would include unique recombinant forms (URFs,38.5%),subtype C (34.6%),CRF01_AE (21.2%),CRF08_BC (3.8%),and subtype B (1.9%). One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in respective two sequences. Based on the statistical method of HIVDR-TS,the prevalence of transmitted HIV-1 drug resistance was adjusted as a moderate level (5%-15%). Conclusion Diverse HIV-1 genotypes were found in this study,and the current HIV-1 drug resistant strains transmission was catalogued as moderate prevalence level in Dehong.

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