| 李世军,刘英,王月,马青,黄艳,周敬祝,余春,田克诚,邹志霆,唐光鹏,王定明.贵州省2010-2012年6株羊种布鲁氏菌分子流行病学特征研究[J].Chinese journal of Epidemiology,2014,35(10):1138-1141 |
| 贵州省2010-2012年6株羊种布鲁氏菌分子流行病学特征研究 |
| Study on the epidemiologic characteristic of Brucella melitensis isolated in Guizhou province in 2010-2012 |
| Received:June 04, 2014 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.10.014 |
| KeyWord: 布鲁氏菌，羊种 分子流行病学 |
| English Key Word: Brucella melitensis Molecular epidemiology |
| FundProject:贵州省科技公关计划社会发展项目（黔科合SY［2013］3049）；贵州省高层次人才科研条件特助经费项目（TZJF-2010-100号）；贵州省科学技术基金项目（黔科合J字［2013］2166号） |
| Author Name | Affiliation | E-mail | | Li Shijun | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | zjumedjun@163.com | | Liu Ying | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Wang Yue | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Ma Qing | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Huang Yan | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Zhou Jingzhu | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Yu Chun | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Tian Kecheng | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Zou Zhiting | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Tang Guangpeng | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | | | Wang Dingming | Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China | |
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| Abstract: |
| 目的 了解贵州省近年羊种布鲁氏菌分离株的分子流行病学特征。方法 应用布鲁氏菌属外膜蛋白31 基因PCR（BCSP31-PCR）和基于插入序列IS711 的牛、羊、绵羊附睾和猪种布鲁氏菌特异的PCR（AMOS-PCR）对贵州省2010-2012 年间分离的6 株布鲁氏菌进行鉴定，采用16 个可变数量串联重复序列（VNTR）的多位点可变数目重复序列分析（MLVA-16）方法对上述菌株进行分型，并做聚类分析。结果 6 株菌经BCSP31-PCR鉴定为布鲁氏菌属细菌、AMOS-PCR鉴定为羊种布鲁氏菌。MLVA-16 分析显示，6 株菌在部分VNTR位点上存在重复数目差异，菌株ZY和ZA为MT63 型，菌株LL3、LL4 和LL11 为MT67 型，菌株SQ为MT72 型。聚类分析显示，6 株菌株中的ZY、ZA、LL3、LL4 和LL9 株与来自云南、福建和广东省羊3 型布鲁氏菌聚类最近，菌株SQ与其他菌株聚类相对较远。结论 2010-2012 年贵州省布鲁氏菌分离株均为羊种生物3 型，菌株间存在重复序列多态性，与来自云南、福建和广东省羊3 型布鲁氏菌聚类最近。 |
| English Abstract: |
| Objective To understand the genetic and epidemiologic characteristic of Brucella （B.） melitensis strains isolated in Guizhou province in 2010-2012. Methods B. genus specific BCSP31-PCR and species-specific AMOS-PCR were used to identify the bacteria strain,while the identified strains were analyzed under MLVA-16 and cluster analysis of B. melitensis strains. The strains were isolated from Guizhou and other provinces. Results Six B. melitensis strains were identified as B. melitensis using the BCSP31-PCR and AMOS-PCR. Data from the MLVA-16 analysis revealed the differences of repeated numbers at parts of the VNTR locus in the six strains isolated in Guizhou province. The six strains from Guizhou province and 105 B. melitensis strains from other province could be divided into 72 MLVA types（MT）. Strain ZY and ZA from Guizhou province were typed as MT63,and LL3,LL4 and LL11 were typed as MT67,while strain SQ was typed as MT72. Data from the clustering analysis showed that ZY,ZA,LL3,LL4 and LL9 were most closely clustered with B. melitensis isolates from Yunnan,Fujian and Guangdong provinces,but strain SQ was genetically remote from other strains. Conclusion PCR methods,combined with MLVA-16, identified the six B. melitensis strains isolated in Guizhou province in 2010-2012 as B. melitensis biovar 3,with the genetic diversity of the strains showed. Six strains were closely related to the B. melitensis strains from Yunnan,Fujian and Guangdong provinces. The results of this study provided scientific basis for the control and prevention of Brucellosis in Guizhou province. |
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