广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究

郑丹; 邓伟; 黄天壬; 李曦亮; 李召发

Abstract

郑丹,邓伟,黄天壬,李曦亮,李召发.广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究[J].Chinese journal of Epidemiology,2015,36(7):725-729

广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究

Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region

Received:December 09, 2014

DOI：10.3760/cma.j.issn.0254-6450.2015.07.013

KeyWord: 肝癌乙型肝炎病毒基因型突变

English Key Word: Hepatocellular carcinoma Hepatitis B virus Genotype Mutation

FundProject:国家自然科学基金(30960333,81260319); 广西壮族自治区高校科技项目(2013ZD016)

Author Name	Affiliation	E-mail
Zheng Dan	Guangxi Medical University
Deng Wei	Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China
Huang Tianren	Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China	tianrenhuang@sina.com
Li Xiliang	Guangxi Medical University
Li Zhaofa	Guangxi Medical University

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Abstract:

目的研究广西壮族自治区(广西)扶绥县肝癌高发区HBV基因型、基本核心启动子(BCP)/前C区突变与肝癌的关系。方法采用病例对照方法收集53例肝癌患者和70例HBV健康携带者的血清,提取HBV DNA,用巢式PCR扩增 HBV S区、BCP/前C区,扩增产物纯化后测序,分析基因型、基因突变与肝癌发生的关系。结果 BCP区A1762T/G1764A及前C区T1858C在病例组中的突变率高于对照组,分别为94.3% vs. 75.7%(P=0.006)和50.9% vs. 31.4%(P=0.029);A1775G在对照组中的突变率高于病例组28.6% vs.13.2%(P=0.041)。多因素logistic回归分析显示,HBV A1762T/G1764A和T1858C突变是肝癌发生的危险因素,OR值分别为5.459(95%CI:1.397~21.332,P=0.015)和3.881(95%CI:1.462~10.305,P=0.006);A1775G突变是肝癌发生的保护因素,OR=0.192(95%CI:0.059~0.622,P=0.006)。结论 HBV C基因型是广西肝癌高发区的主要流行株,HBV BCP区A1762T/G1764A、A1775G、前C区T1858C 突变与HBV相关肝癌的发生有密切关系。

English Abstract:

Objective To investigate the relationship between hepatitis B virus (HBV) genotype, the mutation in basic core promoter(BCP) region/pre-core(Pre-C) region and the incidence of hepatocellular carcinoma(HCC) in Fusui county of Guangxi Zhuang Autonomous Region(Guangxi), a area with high incidence of HCC. Methods In this case-control study, 53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype, gene mutation of HBV and the incidence of HCC was analyzed. Results The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group (94.3% vs. 75.7%, P=0.006; 50.9% vs. 31.4%, P=0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%) (P=0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC(OR=5.459, 95%CI:1.397- 21.332, P=0.015;OR=3.881, 95%CI:1.462-10.305, P=0.006). A1775G is the protective factor in the development of HCC (OR=0.192, 95%CI:0.059-0.622, P=0.006). Conclusion The present investigation showed that BCP A1762T/G1764A, A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.

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