宁夏回族自治区2013年手足口病患者柯萨奇病毒A组10型分离株VP1区基因特征分析

马江涛; 袁芳; 陈慧; 马学旻; 詹军; 李丽

Abstract

马江涛,袁芳,陈慧,马学旻,詹军,李丽.宁夏回族自治区2013年手足口病患者柯萨奇病毒A组10型分离株VP1区基因特征分析[J].Chinese journal of Epidemiology,2015,36(7):734-737

宁夏回族自治区2013年手足口病患者柯萨奇病毒A组10型分离株VP1区基因特征分析

Genetic characteristics of VP1 region of coxsackievirus A10 strains isolated from hand foot and mouth disease patients in Ningxia Hui Autonomous Region, 2013

Received:December 03, 2014

DOI：10.3760/cma.j.issn.0254-6450.2015.07.015

KeyWord: 手足口病柯萨奇病毒A组10型基因特征

English Key Word: Hand foot and mouth disease Coxsackievirus A10 Genetic characteristics

FundProject:宁夏回族自治区科研支撑项目(2013ZYS119)

Author Name	Affiliation	E-mail
Ma Jiangtao	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China	majt1980@sohu.com
Yuan Fang	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China
Chen Hui	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China
Ma Xuemin	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China
Zhan Jun	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China
Li Li	Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China

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Abstract:

目的了解宁夏回族自治区(宁夏)手足口病(HFMD)患者中柯萨奇病毒A组10型(Cox A10)分离株的VP1区基因特征。方法收集2013年real-time PCR 检测非EV71和非Cox A16肠道病毒标本280份,用RD细胞进行肠道病毒分离培养,分离到的毒株采用RT-PCR扩增其VP1区基因并进行核苷酸序列测定,测序结果利用BLAST进行型别鉴定。对鉴定为Cox A10的所有毒株进行VP1区基因同源性分析和进化分析。结果从280份标本中共分离到肠道病毒36株,其中有6株鉴定为Cox A10,核苷酸和氨基酸同源性分别为97.0%~99.8%和99.0%~99.7%。与A、B、C、D各基因亚型代表株之间的核苷酸同源性分别为76.3%~77.2%、81.6%~83.1%、94.4%~98.9%和80.0%~82.3%,氨基酸序列同源性分别为92.3%~93.0%、94.0%~95.3%、98.0%~99.7%和90.6%~94.0%。系统进化显示,6株Cox A10宁夏株与C基因型代表株处于同一分支。结论 Cox A10是引起宁夏2013年HFMD的常见病原体,本次分离到的Cox A10均属于C基因型。

English Abstract:

Objective To study the genetic characteristics of VP1 region of coxsackievirus A10(Cox A10) strains isolated from hand foot and mouth disease (HFMD) cases in Ningxia Hui Autonomous Region (Ningxia) in 2013. Methods A total of 280 specimens, which were identified as non-enterovirus 71 and non-Cox A16 by real-time PCR, were collected and cultured by using RD cell, and the VP1 genes of isolated strains were amplified by using reverse transcriptase PCR(RT-PCR) with degenerated primers and sequenced. The sequencing results were aligned with the sequences in GenBank with BLAST algorithm to identify the virus genotypes. Homologous comparison and phylogenetic analysis were conducted for all the Cox A10 strains identified. Results Among 36 virus strains isolated from 280 clinical specimens, 6 were identified as Cox A10.The homologies of nucleotide and amino acid of the Cox A10 strains isolated in Ningxia were 97.0%-99.8% and 99.0% -99.7% respectively, and the Cox A10 strains isolated in Ningxia shared 76.3%-77.2%, 81.6%-83.1%, 94.4%-98.9% and 80.0%- 82.3% nucleotide homologies respectively and shared 92.3%-93.0%, 94.0%-95.3%, 98.0%-99.7% and 90.6%-94.0% amino acid homologies respectively with the representative strains of A, B, C and D genotypes. Phylogenetic tree analysis revealed that Cox A10 strains isolated in Ningxia belonged to genotype C. Conclusion Cox A10 is one of the most common pathogen causing HFMD in Ningxia in 2013. All the Cox A10 stains isolated from HFMD patients in Ningxia belonged to genotype C.

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