锌转运蛋白-8基因单核苷酸多态性和基因-吸烟的交互作用及与维吾尔族人群2型糖尿病的关联研究

苏银霞; 王志强; 姚华; 王婷婷; 马琦; 朱筠; 王淑霞; 马艳

Abstract

苏银霞,王志强,姚华,王婷婷,马琦,朱筠,王淑霞,马艳.锌转运蛋白-8基因单核苷酸多态性和基因-吸烟的交互作用及与维吾尔族人群2型糖尿病的关联研究[J].Chinese journal of Epidemiology,2015,36(10):1167-1171

锌转运蛋白-8基因单核苷酸多态性和基因-吸烟的交互作用及与维吾尔族人群2型糖尿病的关联研究

Association between type 2 diabetes in Uygur and polymorphisms of SLC30A8 and its interaction with smoking

Received:February 10, 2015

DOI：10.3760/cma.j.issn.0254-6450.2015.10.028

KeyWord: 糖尿病,2型锌转运蛋白-8基因单核苷酸多态性吸烟交互作用

English Key Word: Type 2 diabetes Solute carrier family 30,member 8 gene Single-nucleotide polymorphism Smoking Interaction

FundProject:国家重点基础研究发展计划(973计划)(2012CB722403);新疆重大疾病医学重点实验室开放课题(SKLIB-XJMDR-2012-Y4,SKLIB-XJMDR-2014-Y4);新疆医科大学第一附属医院科研基金(2013ZRQN18)

Author Name	Affiliation	E-mail
Su Yinxia	Key Laboratory of Metabolic Diease, the First Affiliated Hospital, 2 Department of Public Health, Xinjiang Medical University, Urumqi 830011, China
Wang Zhiqiang	公共卫生学院
Yao Hua	Key Laboratory of Metabolic Diease, the First Affiliated Hospital, 2 Department of Public Health, Xinjiang Medical University, Urumqi 830011, China	yaohua01@sina.com
Wang Tingting	公共卫生学院
Ma Qi	Key Laboratory of Metabolic Diease, the First Affiliated Hospital, 2 Department of Public Health, Xinjiang Medical University, Urumqi 830011, China
Zhu Jun	Key Laboratory of Metabolic Diease, the First Affiliated Hospital, 2 Department of Public Health, Xinjiang Medical University, Urumqi 830011, China
Wang Shuxia	Key Laboratory of Metabolic Diease, the First Affiliated Hospital, 2 Department of Public Health, Xinjiang Medical University, Urumqi 830011, China
Ma Yan	Shayibake District Centers for Disease Control and Prevention, Urumqi

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Abstract:

目的探讨锌转运蛋白-8(SLC30A8)基因单核苷酸多态性和基因-吸烟交互作用及与维吾尔(维)族人群T2DM的关联性。方法采用病例-对照研究方法以1 000例确诊为T2DM者作为病例组,按照与病例组同民族年龄相差±3岁的原则选取1 010例为对照组。收集研究对象血液标本5 ml,提取基因组DNA。应用基质辅助激光解吸附电离飞行时间质谱分析技术(MALDI-TOF)检测SLC30A8的单核苷酸多态性,采用SPSS 16.0统计软件分析数据。结果 SLC30A8的rs13266634位点等位基因频率在两组间的差异有统计学意义,C为危险等位基因OR=1.194(95%CI:1.044 ~1.366);rs13266634基因型分布频率在调整被动吸烟、主动吸烟、SBP、TC、HDL-C、LDL-C、BMI后,两组间差异均有统计学意义(P<0.05)。基因模型分析显示rs13266634在显性模型中有意义(OR=1.640,95%CI:1.072~2.510)。病例组中被动吸烟所占比例高于对照组,主动吸烟两组间差异无统计学意义(P>0.05)。rs13266634与主动吸烟及被动吸烟的乘积项均无统计学意义(P>0.05),二者对维族T2DM均无相乘交互作用;rs13266634与主动吸烟及被动吸烟的相加交互作用指标RERI、AP和S及其95%CI分别为0.301(-1.314~0.712)、0.204(-0.854~0.446)、0.612(0.186~2.013)和0.125(-0.805~1.055)、0.052(-0.353~0.456)、1.096(0.500~2.403),说明对维族T2DM无相加交互作用。结论 SLC30A8的rs13266634位点单核苷酸多态性与维族T2DM有关联性,TT为保护型基因型,rs132666334位点突变可能降低维族人群患T2DM的风险;CC、CT基因型及被动吸烟可能增加维族人群患T2DM的风险。

English Abstract:

Objective To explore the relationship between the polymorphism of solute carrier family 30,member 8 (SLC30A8) gene and type 2 diabetes mellitus (T2DM) in Uyhgur in Xinjiang and further analyze the interaction between SLC30A8 gene polymorphism loci and smoking. Methods A case control study,including 1 000 patients with T2DM and 1 010 non-diabetic controls,was conducted in Xinjiang. All the subjects were Uygur and the age difference between the two groups was within 3 years. Physical examination and blood biochemical detection were performed to obtain personal clinical parameters. Genomic DNA was extracted from peripheral blood leukocytes. The single nucleotide polymorphism(SNP) of SLC30A8 of all the subjects was tested by using MALDI-TOF. Statistical analyses were performed with SPSS 16.0. Bootstrap method was used to calculate 95% confidence intervals of RERI,AP and S. Results After adjusting BMI,SBP,TC,HDL-C and LDL-C,rs13266634 of SLC30A8 gene genotype frequency and allele frequency distribution had statistical differences (P<0.05). Rs13266634 of risk allele were C,OR was 1.194 (95%CI:1.044-1.366). In addition,the data from genotype distribution analysis under different models showed that significant association between rs13266634 and T2DM in dominant model,OR was 1.640 (95%CI 1.072-2.510). The product of rs13266634 with the active smoking or passive smoking had no statistical significance (P>0.05),indicating there were no multiplication interaction among them. Additive interactions index of RERI,AP and S and its 95% confidence interval of rs13266634 and active smoking,rs13266634 and passive smoking were 0.301(-1.314-0.712),0.204 (-0.854-0.446),0.612(0.186-2.013) and 0.125(-0.805-1.055),0.052(-0.353-0.456),1.096 (0.500-2.403) respectively,indicating there were no significant additive interaction among them. Conclusion Rs13666334 of SLC30A8 gene is associated with the susceptibility of T2DM in Uygur,and its protective genotype might be TT. Passive smoking might increase the risk of T2DM in Uygur.

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