中国土拉弗朗西斯菌holarctica亚种的遗传多样性

王艳华; 彭遥; 夏连续

Abstract

王艳华,彭遥,夏连续.中国土拉弗朗西斯菌holarctica亚种的遗传多样性[J].Chinese journal of Epidemiology,2015,36(12):1410-1414

中国土拉弗朗西斯菌holarctica亚种的遗传多样性

Genetic diversity of Francisella tularensis subsp. holarctica in China

Received:July 02, 2015

DOI：10.3760/cma.j.issn.0254-6450.2015.12.021

KeyWord: 土拉弗朗西斯菌土拉热系统进化单核苷酸多态性插入/缺失多位点可变串联重复分析

English Key Word: Francisella tularensis Tularemia Phylogeography Single-nucleotide polymorphism Insertion-deletion Multiple-locus variable number tandem repeat analysis

FundProject:国家自然科学基金(81473032); 国家科技重大专项(2012ZX10004219-007)

Author Name	Affiliation	E-mail
Wang Yanhua	National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China	wangyanhua@icdc.cn
Peng Yao	National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xia Lianxu	National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

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Abstract:

目的研究国内外土拉弗朗西斯菌的遗传进化关系。方法选择17个单核苷酸多态性、4个插入/缺失和12个可变数目串联重复,采用单核苷酸多态性和插入/缺失、多位点可变数目串联重复分析方法单独和组合起来对39株土拉菌(10株中国土拉菌和29株已公布测序的土拉菌)进行系统进化分析。结果组合分析显示,3株中国土拉菌和日本的FSC022被分配到B5;剩余3株中国土拉菌和瑞典的FSC200被分配到B1;3株和美国的OSU18被分配到B2;1株和法国的FTNF002-00、德国的F92与美国的OR96246一起被分配到B4。10株中国土拉菌分为4种亚型,研究表明中国土拉菌具有广泛的遗传多样性。结论本研究针对土拉菌B型建立了一套简易高效的分型方法,并以此为基础得出土拉菌B型的起源可能是亚洲地区。

English Abstract:

Objective To explore the genetic relationship between the Chinese and the foreign species of Francisella tularensis. Methods Based on our own findings and from the literature, 17 SNP, 4 INDEL, and 12 VNTR were selected for phylogenetic analysis on 39 strains of F. tularensis, including 10 strains of Chinese F. tularensis and 29 strains of foreign F. tularensis that had been sequenced and published. SNP-INDEL and MLVA were used for the separation and combination. Results Data from the combined analysis indicated that 3 strains of Chinese F. tularensis with Japanese FSC022 were assigned to B5; 3 strains, with Swedish FSC200 to B1; 3 strains with American OSU18 to B2 and 1 strain with French FTNF002-00, German F92, and American OR96246 to B4, respectively. 10 strains of Chinese F. tularensis were assigned to 4 clades and the result demonstrated a wide diversity of F. tularensis subsp. holarctica in China. Conclusion A set of simple and robust typing tools for F. tularensis subsp. holarctica were established in this study. Based on the results, F. tularensis subsp. holarctica might have had its origins in Asia.

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