| 崔大伟,李中杰,林洁,靳淼,杨先知,谢国良,郑书发,余斐,陈瑜.杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析[J].Chinese journal of Epidemiology,2016,37(2):254-258 |
| 杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析 |
| Epidemiologic characteristics of noroviruses isolated in outpatients with acute gastroenteritis in Hangzhou area, from 2014 to 2015 |
| Received:August 17, 2015 |
| DOI：10.3760/cma.j.issn.0254-6450.2016.02.021 |
| KeyWord: 诺如病毒 急性胃肠炎 聚合酶链反应 基因型 |
| English Key Word: Noroviruses Acute gastroenteritis Polymerase chain reaction Genotype |
| FundProject:国家科技重大专项(2012ZX10004-210) |
| Author Name | Affiliation | E-mail | | Cui Dawei | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Li Zhongjie | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Lin Jie | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Jin Miao | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Yang Xianzhi | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Xie Guoliang | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China | | | Zheng Shufa | Division of Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, State Key Laboratory for Communicable Disease Surveillance and Early Warning, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Yu Fei | Department of Clinical Laboratory, Center of Community Health Service of Qingbo Street, Hangzhou 310002, China | | | Chen Yu | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | chenyu_zy@163.com |
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| Abstract: |
| 目的 了解2014-2015年杭州地区急性胃肠炎患者中诺如病毒的流行病学特征。方法 收集1 109份急性胃肠炎患者的临床流行病学资料和粪便样本,采用一步法双重荧光RT-PCR对样本中诺如病毒(GⅠ、GⅡ型)进行初筛,然后随机选择诺如病毒阳性的样本核酸进行多重RT-PCR扩增,对产物进行测序分析。结果 1 109份的粪便样本中,诺如病毒检出率为26.87%(298/1 109),以GⅡ亚型为主,占25.52%(283/1 109),GⅠ亚型占1.35%(15/1 109);不同性别人群诺如病毒检出率差异无统计学意义(P>0.05);不同年龄组人群中诺如病毒检出率分别为16.94%(<5岁)、19.45%(5~18岁)和32.26%(≥18岁);全年均可检出诺如病毒感染,高发季节为12月至次年3月,阳性率为29.67%~37.08%。序列分析显示,GⅡ基因亚型中以GⅡ-4/Sydney株为主(46.43%,13/28),其次为GⅡ-4/2006b株;GⅠ基因亚型中以GⅠ-1亚型为主(55.56%,5/9)。结论 诺如病毒是导致2014-2015年杭州地区急性胃肠炎的主要病原之一,GⅡ基因亚型特别是GⅡ-4/Sydney变异株和GⅡ-4/2006b变异株是该地区流行的优势毒株。 |
| English Abstract: |
| Objective To explore the epidemiologic characteristics of noroviruses isolated in patients with acute gastroenteritis in Hangzhou between March 2014 and April 2015.Methods Stool specimens and clinical data were collected from 1 109 patients with acute gastroenteritis.Specimens were detected for noroviruses with GⅠ and GⅡ subtypes by one-step double real-time RT-PCR.Some of the positive specimens were then randomly selected and amplified by multiplex RT-PCR.Those positive PCR products were sequenced and analyzed phylogenetically for testing the partial capsids of noroviruses.Results Of the 1 109 stool specimens, positive rate of noroviruses was 26.87%(298/1 109).GⅡ genotype was the major viruses with the proportion as 25.52%(283/1 109), while 1.35%(15/1 109) belonged to GⅠ genotypes.There was no significant difference in the noroviruses detection rate of the different genders(P>0.05).However, in different age groups, GⅡ genotypes were predominant types of noroviruses, and the positive rates of GⅡ genotypes were 16.94%(<5 years-old), 19.45%(5-18 years-old) and 32.26%(≥18 years-old), respectively.In different seasons, noroviruses could be detected all year round, with positive rate as 29.67%-37.08% in the highly epidemic seasons(between December and March of the following year).The distribution trends were seen certain difference between noroviruses-GⅡ and GⅠ types.Additionally, Results from the sequence analysis demonstrated that GⅡ-4 genotype was the prevalent strain of GⅡ genotypes, clustered into GⅡ-4/Sydney(46.43%, 13/28) and GⅡ-4/2006b(25.0%, 7/28), while G Ⅰ strains clustered into GⅠ-1.Conclusion Noroviruses appeared one of the major pathogens, leading to acute gastroenteritis.GⅡ genotypes of noroviruses, especially the GⅡ-4/Sydney variant strains and GⅡ-4/2006b variant strains, were considered to be the prevalent strains prevailed in Hangzhou areas from 2014 to 2015. |
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