类鼻疽伯克霍尔德菌跨洲际流行株的同源性分析及历史溯源

郑霄; 王鲁茜; 吴华; 陈海; 朱雄; 贺金荣; 夏连续; 李伟

Abstract

郑霄,王鲁茜,吴华,陈海,朱雄,贺金荣,夏连续,李伟.类鼻疽伯克霍尔德菌跨洲际流行株的同源性分析及历史溯源[J].Chinese journal of Epidemiology,2017,38(5):661-664

类鼻疽伯克霍尔德菌跨洲际流行株的同源性分析及历史溯源

Homology analysis and historical tracing for inter-continental Burkholderia pseudomallei strains of sequence type 562

Received:October 15, 2016

DOI：10.3760/cma.j.issn.0254-6450.2017.05.021

KeyWord: 类鼻疽伯克霍尔德菌跨洲际流行同源性分析

English Key Word: Burkholderia pseudomallei Inter-continental epidemic Homology analysis

FundProject:国家自然科学基金（81573208）；海南省自然科学基金（814389）

Author Name	Affiliation	E-mail
Zheng Xiao	State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wang Luxi	Emergency Office, Changping District Center for Disease Control and Prevention, Beijing 102200, China
Wu Hua	Department of Laboratory, Hainan Provincial People's Hospital, Haikou 570311, China
Chen Hai	Department of Laboratory, Sanya People's Hospital, Sanya 572000, China
Zhu Xiong	Department of Laboratory, Sanya People's Hospital, Sanya 572000, China
He Jinrong	State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xia Lianxu	State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Li Wei	State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China	liwei@icdc.cn

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Abstract:

目的比较不同年代分离自澳大利亚、中国海南地区7株ST562型类鼻疽伯克霍尔德菌（类鼻疽伯克菌）的同源性，并分析其传播来源。方法利用生物信息学方法提取ST562型类鼻疽伯克菌澳大利亚临床株MSHR5858、中国海南历史菌株350105基因组中Spe Ⅰ限制性酶切片段指纹谱、多位点可变数目串联重复序列多态性指纹（MLVA-4）等遗传特征，并与近年分离的5株海南ST562型临床株的脉冲场凝胶电泳（PFGE，Spe Ⅰ酶切）、MLVA-4分子分型结果相比较。同时分析MSHR5858与350105在基因组水平的共线性及同源性。结果 5株海南ST562型临床株的PFGE带型相同（相似度>97%）且与澳大利亚临床株MSHR5858的Spe Ⅰ限制性带型一致；澳洲临床株（MSHR5858）与海南历史菌株（350105）基因组中Spe Ⅰ限制片段数分别为31和34，其中31个片段的长度一致。5株海南ST562临床株的MLVA-4型别各不相同，但HPPH43（MLVA-4指纹谱：10，8，10，8）与MSHR5858（10，8，8，6）在2341 k、1788 k两个位点重复数相同；HK003（11，8，15，7）、HK061（11，8，17，7）与历史菌株350105（11，8，11，8）在此二位点重复数也相同。此外，350105与MSHR5858在基因组水平具有良好共线性，共有基因占绝大部分，提示来源一致。结论分离的7株ST562型类鼻疽伯克菌（包括中国海南临床分离株、历史菌株及澳大利亚临床分离株）遗传特征一致，且ST562型近年流行株与历史菌株350105可能具有同源关系。

English Abstract:

Objective To understand the homology of sequence type 562 (ST562) strains of Burkholderia pseudomallei which circulated in two separate continents (Asia and Australia) at different times. Methods Spe Ⅰrestriction fragments and 4-locus multiple locus variable number tandem repeat analysis (MLVA-4) profiles were extracted from MSHR5858 (ST562 Australia strain) and 350105 (ST562 historical strain of Hainan) genomes respectively by in silico analysis and then compared with the PFGE and MLVA-4 results of five ST562 clinical isolates from Hainan to test their homology. Synteny and homology between MSHR5858 and 350105 genomes were evaluated with bioinformatics methods. Results Five ST562 clinical strains from Hainan shared same PFGE pattern (similarity >97%) and this pattern coincided to the map of Spe Ⅰrestriction fragments of Australian strain MSHR5858. The amounts of genomic restriction fragments (Spe Ⅰ) for MSHR5858 and 350105 were 31 and 34 respectively, with 31 of them matched by each other. Five ST562 clinical strains of Hainan were distinct by MLVA-4 profiles, among which HPPH43 (MLVA-4 profile: 10, 8, 10, 8) was close to Australia strain MSHR5858 (10, 8, 8, 6), containing identical repeat numbers at VNTR loci 2341k and 1788k; while HK003 (11, 8, 15, 7) and HK061 (11, 8, 17, 7) similar to Hainan historical strain 350105 (11, 8, 11, 8), with same repeat numbers at loci 2341k and 1788k also. High-degree synteny and consistency on genomic contents were observed between 350105 and MSHR5858, indicating a similar origin for the 2 strains. Conclusion All inter-continental and historical ST562 strains of B. pseudomallei had similar genomic characteristics, supporting the assumption that they had a common origin. Also, it is possible that Hainan historical strain 350105 is the ancestor of all circulating ST562 strains.

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