| 刘志国,王妙,刘日宏,尚修建,崔步云.内蒙古自治区乌兰察布市羊种布鲁氏菌HOOF基因分型特征[J].Chinese journal of Epidemiology,2017,38(7):954-958 |
| 内蒙古自治区乌兰察布市羊种布鲁氏菌HOOF基因分型特征 |
| HOOF genotyping characteristics of Brucella melitensis strains isolated in Ulanqab of Inner Mongolia Autonomous Region |
| Received:November 29, 2016 |
| DOI：10.3760/cma.j.issn.0254-6450.2017.07.021 |
| KeyWord: 布鲁氏菌病 羊种布鲁氏菌 HOOF基因分型 |
| English Key Word: Brucellosis Brucella melitensis HOOF genotyping |
| FundProject:内蒙古自治区卫生计划生育委员会医疗卫生科研计划（201301094） |
| Author Name | Affiliation | E-mail | | Liu Zhiguo | Department of Laboratory, Ulanqab Center for Endemic Disease Prevention and Control, Ulanqab 012000, China | | | Wang Miao | Department of Laboratory, Ulanqab Center for Endemic Disease Prevention and Control, Ulanqab 012000, China | | | Liu Rihong | Department of Laboratory, Ulanqab Center for Endemic Disease Prevention and Control, Ulanqab 012000, China | | | Shang Xiujian | Department of Brucellosis, Xinjiang Uygur Autonomous Region Center for Diseases Control and Prevention, Urumqi 830001, China | | | Cui Buyun | Department of Brucellosis, National Institute of Communicable Diseases Prevention and Control, Chinese Center for Disease Prevention and Control, State Key Laboratory for Communicable Disease Prevention and Control, Beijing 102206, China | cuibuyun@icdc.com |
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| Abstract: |
| 目的 调查2012-2015年内蒙古自治区乌兰察布市83株布鲁氏菌HOOF分型特征。方法 对临床分离的83株布鲁氏菌采用常规方法和AMOS-PCR进行鉴定；利用8个数目可变串联重复序列 位点的HOOF分型方法对菌株进行基因分型，并用Hunter-Gaston 分辨指数评估各VNTR 位点的多态性和对菌株的综合辨别能力，采用BioNumerics 5.0软件聚类分析和构建聚类图。结果 83株试验菌全部为羊种布鲁氏菌，全部8个分型位点具有极高的多态性，多态性指数为0.998；其中6个位点（1、2、4~7）的多态性指数 ≥ 0.678，其分辨力主要来自多态性指数较高的位点。83株羊种布鲁氏菌聚为8大类76个基因型。6个共享基因型包括13株布鲁氏菌，提示感染患者具有流行病学相关性；另70株菌呈现独特的基因型，提示病原分离自无流行病学相关的散发患者。结论 乌兰察布市人布鲁氏菌病流行以散发和局部暴发共存，且散发为主，而交叉感染患者与传染源转移有关。 |
| English Abstract: |
| Objective To investigate the HOOF genotyping characteristics of 83 Brucella (B.) melitensis strains isolated in Ulanqab of Inner Mongolia Autonomous Region from 2012 to 2015. Methods A total of 83 B. melitensis strains were detected by convention identification and AMOS-PCR, then HOOF protocol with eight VNTR locus were used for the genotyping of the strains, and the allelic diversity of each VNTR locus and the discriminatory power of VNTR typing of HOOF were assessed by Hunter-Gaston Discriminatory index. BioNumerics 5.0 was used for phylogenetic analysis and constructing dendrogram. Results All of the isolates were identified as B. melitensis strains by two identification methods. The complete eight VNTR locus had higher polymophism and diversity index was 0.998; and diversity index of six locus (1, 2 and 4-7) were ≥ 0.678, discriminatory power of HOOF was mainly from this six higher diversity index locus. The 83 B. melitensis strains were classified into eight clusters and 76 genotypes, 6 shared genotypes included 13 isolates, indicating that these brucellosis cases had epidemiological link, the other 70 strains had distinct genotypes, indicating that these cases had no epidemiological link. Conclusions The epidemic of human brucellosis in Ulanqab was characterized by local and sporadic outbreaks. Cross infection was related with the transfer of the sources of infection. |
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