| 段星,王可然,王继宝,杨涛,王译葵,杨锦,叶润华,杨跃诚,姚仕堂,段松,何纳.云南省德宏傣族景颇族自治州2015年新报告HIV感染者亚型分析[J].Chinese journal of Epidemiology,2017,38(8):1107-1112 |
| 云南省德宏傣族景颇族自治州2015年新报告HIV感染者亚型分析 |
| HIV subtype in newly reported HIV infected cases in Dehong prefecture of Yunnan province,2015 |
| Received:November 25, 2016 |
| DOI：10.3760/cma.j.issn.0254-6450.2017.08.022 |
| KeyWord: 艾滋病病毒 亚型分布 中国籍 缅甸籍 |
| English Key Word: Human immunodeficiency virus Subtype distribution Chinese Burmese |
| FundProject:国家重点地区艾滋病防治项目；国家科技重大专项（2013ZX10004-906）；国家自然科学基金（81373062） |
| Author Name | Affiliation | E-mail | | Duan Xing | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Wang Keran | Department of Epidemiology, School of Public Health, The Key Laboratory for Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China | | | Wang Jibao | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Yang Tao | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Wang Yikui | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Yang Jing | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Ye Runhua | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Yang Yuecheng | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Yao Shitang | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | | | Duan Song | Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention, Mangshi 678400, China | dhduansong@sina.com.cn | | He Na | Department of Epidemiology, School of Public Health, The Key Laboratory for Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China | nhe@shmu.edu.cn |
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| Abstract: |
| 目的 分析云南省德宏傣族景颇族自治州（德宏州）2015年1-11月新报告HIV感染者亚型，掌握德宏州中国籍和缅甸籍HIV感染者亚型分布特征。方法 对德宏州2015年1-11月新报告的HIV感染者且血浆量在200 μl以上的标本进行核酸抽提，使用RT-PCR方法对HIV的gag、env和pol 3个基因进行反转录扩增，所得产物直接测序，确定亚型型别。结果 2015年1-11月德宏州新报告HIV感染者963例，成功测定HIV亚型的感染者499例。独特重组（URFs）比例最大（27.1%，135/499）；其后为C（26.7%，133/499）和CRF01_AE（19.2%，96/499）等亚型。URFs中主要包括4种形式的重组，其中以BC亚型重组最多。中国籍和缅甸籍感染者的亚型分布特征不同，传播方式是唯一与中国籍和缅甸籍感染者亚型分布有关联的因素。结论 德宏州中国籍和缅甸籍HIV感染者亚型分布复杂，URFs首次居分布首位。中国籍和缅甸籍感染者亚型分布有差异，但主要受HIV传播方式的影响。 |
| English Abstract: |
| Objective To explore the distribution of HIV subtype in newly detected people living with HIV from January to November, 2015 in Dehong Dai and Jingpo Autonomous Prefecture, Yunnan province. Methods DNA extraction, reverse transcription polymerase chain reaction (RT-PCR) for gag, env, and pol amplification and amplification product sequencing were conducted by using plasmas of newly detected HIV-infected persons. The subtypes were confirmed by analyzing the sequences of 3 genes. Results A total of 963 HIV infection cases were reported during this period, the HIV subtype was confirmed in 499 cases. Unique recombinant form (URF) was the most common subtype (27.1%, 135/499), followed by C (26.7%, 133/499), CRF01_AE (19.2%, 96/499) and others. URF included 4 kinds of combination, of which combination of subtype B and C was most common. HIV subtype distribution differed between the Chinese HIV infection cases and the Burmese HIV infection cases, the proportion of B and C combination was higher in the Chinese cases. Transmission route was the only factor influencing HIV subtype distribution. Conclusions HIV subtype distribution in Dehong was complex. URF was predominant. The HIV subtype distribution differed between Chinese and Burmese under different transmission route. |
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