| 李柏生,陈柳军,柯碧霞,林洁敏,徐励琴,谭海玲,何冬梅,梁宇恒,柯昌文,张永慧.广东省和广西壮族自治区部分地区2014-2016年宋内志贺菌病原学特征分析[J].Chinese journal of Epidemiology,2017,38(11):1541-1545 |
| 广东省和广西壮族自治区部分地区2014-2016年宋内志贺菌病原学特征分析 |
| Etiologic characteristics of Shigella sonnei strains isolated from some areas of Guangdong province and Guangxi Zhuang Autonomous Region of China, 2014-2016 |
| Received:May 02, 2017 |
| DOI：10.3760/cma.j.issn.0254-6450.2017.11.021 |
| KeyWord: 宋内志贺菌 病原学特征 脉冲场凝胶电泳 全基因组测序 |
| English Key Word: Shigella sonnei Etiology characteristic Pulsed-field gel electrophoresis Whole genome sequencing |
| FundProject:广东省科技计划项目（2014A020219004） |
| Author Name | Affiliation | E-mail | | Li Baisheng | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | Chen Liujun | Microbiology Department, Liuzhou Prefecture Center for Disease Control and Prevention, Liuzhou 545007, China | | | Ke Bixia | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | Lin Jiemin | Microbiology Department, Shantou Prefecture Center for Disease Control and Prevention, Shantou 515041, China | | | Xu Liqin | Microbiology Department, Huizhou Prefecture Center for Disease Control and Prevention, Huizhou 516001, China | | | Tan Hailing | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | He Dongmei | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | Liang Yuheng | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | Ke Changwen | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | | | Zhang Yonghui | Microbiology Department, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China | zyh@cdcp.org.cn |
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| Abstract: |
| 目的 了解2014-2016年广东省和广西壮族自治区部分地区宋内志贺菌暴发分离株及散发分离株的病原学特征。方法 对2014-2016年广东省和广西壮族自治区柳州市分离的14株宋内志贺菌暴发菌株和6株散发菌株进行抗生素敏感性试验和PFGE分析，并选择其中6株代表菌株进行全基因组测序，与NCBI上获取的51株国内外宋内志贺菌的基因组进行遗传进化分析。结果 抗生素敏感性检测结果显示，试验菌株对氨苄西林、四环素、庆大霉素、复方新诺明和萘啶酸有较高的耐药性，对阿奇霉素、氯霉素和亚胺培南完全敏感。PFGE分子分型显示，不同地区不同来源的分离株之间PFGE指纹图谱有很高的相似度（93.2%），基于全基因组的遗传进化分析显示，广东省和广西壮族自治区柳州市的宋内志贺菌分离株同处在一个进化分支上，与来自韩国的菌株亲缘关系最为接近。结论 广东省和广西壮族自治区部分地区分离的宋内志贺菌对常用抗生素具有较高的耐药性，对阿奇霉素、氯霉素和亚胺培南有较高的敏感性。试验菌株之间具有相似的PFGE指纹图谱，遗传进化关系相近。 |
| English Abstract: |
| Objective To investigated the etiologic characteristics of Shigella (S.) sonnei strains causing outbreaks and sporadic cases in some areas of Guangdong province and Guangxi Zhuang Autonomous Region during 2014-2016. Methods Fourteen S. sonnei strains isolated from outbreaks and 6 S. sonnei strains from sporadic cases from Guangdong and Liuzhou of Guangxi Zhuang Autonomous Region were tested for antimicrobial resistance and analyzed by pulsed-field gel electrophoresis (PFGE). Six typical strains were selected for whole genome sequencing typing and compared with 51 strains isolated both at home and abroad from NCBI genome database. Results The antibiotic resistance test indicated the isolates had high resistance rate to ampicillin, tetracycline, gentamicin, trimethoprim/sulfamethoxazole and nalidixic acid, while sensitive to azithromycin, chloromycetin and imipenem. PFGE showed high similarity (93.2%) among the strains isolated from different areas. The whole genome sequencing analysis also revealed that all the typical strains were clustered into a same evolution branch, close to some strains from Korea. Conclusions The S. sonnei strains isolated from some areas of Guangdong and Guangxi Zhuang Autonomous Region showed high resistance to commonly used antibiotics, but they were sensitive to azithromycin, chloramphenicol and imipenem. The isolates in this study also showed similar PFGE patterns and close phylogenic evolution. |
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