曹志强,杨文敏,朱秋映,蓝光华,沈智勇,梁淑家,李剑军,陈欢欢,冯毅,何翔,廖玲洁,邢辉,阮玉华,邵一鸣.柳州市1998-2012年新发现HIV基因亚型变化及HIV感染者首次CD4+T淋巴细胞计数比较[J].Chinese journal of Epidemiology,2019,40(5):580-584 |
柳州市1998-2012年新发现HIV基因亚型变化及HIV感染者首次CD4+T淋巴细胞计数比较 |
HIV genetic subtypes and comparison of the first CD4+ T cell counts in newly diagnosed HIV infected patients in Liuzhou, 1998-2012 |
Received:November 14, 2018 |
DOI:10.3760/cma.j.issn.0254-6450.2019.05.017 |
KeyWord: HIV-1 基因亚型 CD4+T淋巴细胞计数 |
English Key Word: HIV-1 Genetic subtypes CD4+T cell counts |
FundProject:广西医药卫生自筹经费计划课题(Z20170126);广西壮族自治区科学技术厅(桂科AB16380213);国家自然科学基金(81460510,81360442);广西八桂学者艾滋病防控关键技术岗位专项 |
Author Name | Affiliation | E-mail | Cao Zhiqiang | National Center for ADIS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Yang Wenmin | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Zhu Qiuying | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Lan Guanghua | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Shen Zhiyong | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Liang Shujia | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Li Jianjun | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Chen Huanhuan | Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China | | Feng Yi | National Center for ADIS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | He Xiang | Guangdong Provincial Institute of Public Health, Guangzhou 511430, China | | Liao Lingjie | National Center for ADIS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Xing Hui | National Center for ADIS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Ruan Yuhua | National Center for ADIS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | ruanyuhua92@163.com | Shao Yiming | Peking University Health Science Center, Beijing 100191, China | yshao@bjmu.edu.cn |
|
Hits: 3249 |
Download times: 1363 |
Abstract: |
目的 分析柳州市1998-2012年新发现HIV基因亚型变化,比较HIV感染者首次CD4+T淋巴细胞计数(CD4),为艾滋病防治提供参考依据。方法 选取我国艾滋病综合防治信息系统中1998-2012年柳州市新发现HIV感染者,对其血浆进行RNA提取、扩增、测序和分型。采用Coharan-Armitage趋势检验法分析HIV基因亚型变化的趋势,采用Wilcoxon秩和检验法分析比较HIV感染者的首次CD4。结果 研究对象共有1 877例HIV感染者。1998-2012年,CRF01_AE、CRF01_AE(Cluster 1)和CRF07_BC亚型构成比分别从78.4%(76/97)上升至91.5%(1 441/1 574)、63.9%(62/97)上升至74.0%(1 164/1 574)和17.5%(17/97)下降至4.6%(72/1 574),趋势性检验差异均有统计学意义(Z=4.632,P<0.001;Z=2.455,P=0.014;Z=-5.943,P<0.001)。CRF01_AE(Cluster 1)、CRF01_AE(Cluster 2)、CRF07_BC和CRF08_BC亚型HIV感染者首次CD4的中位数及四分位数间距分别是230(83~375)、215(48~351)、365(254~503)和334(206~479)个/μl。CRF01_AE(Cluster 1)和CRF01_AE(Cluster 2)亚型HIV感染者的首次CD4均Z=-4.795,P<0.001;Z=-4.238,P<0.001)。结论 1998-2012年柳州市新发现HIV感染者以CRF01_AE亚型为主并呈上升趋势,其首次CD4较低,且新发现CRF01_AE亚簇的种类在增加,其亚簇构成比存在变化趋势。 |
English Abstract: |
Objective To analyze the change trend of HIV genetic subtypes and compare the first CD4+T cell counts of newly diagnosed HIV infected patients in Liuzhou from 1998 to 2012, and provide a reference for AIDS prevention and control. Methods Newly diagnosed HIV-infected patients from 1998 to 2012 in Liuzhou were selected through national HIV/ADIS comprehensive response information management system. Their plasma samples were used for RNA gene extraction, amplification, sequencing and genotyping. Coharan-Armitage trend test was used to analyze the ratio trend of genetic subtypes and phylogenetic clusters of HIV and Wilcoxon Rank Sum Test was used to compare the first CD4+T cell counts (CD4) of the different subtype HIV infected patients. Results A total of 1 877 newly diagnosed HIV infected patients were included in the study. From 1998 to 2012, the proportions of CRF01_AE and CRF01_AE (Cluster 1) increased from 78.4% (76/97) to 91.5% (1 441/1 574), from 63.9% (62/97) to 74.0% (1 164/1 574), and the proportion of CRF07_BC decreased from 17.5% (17/97) to 4.6% (72/1 574), respectively (Z=4.632, P<0.001; Z=2.455, P=0.014; Z=-5.943, P<0.001). The median and interquartile range of the first CD4 of the patients infected with subtype CRF01_AE (Cluster 1), CRF01_AE (Cluster 2), CRF07_BC and CRF08_BC were 230 (83-375), 215 (48-351), 365 (254-503) and 334 (206-479) cell/μl, respectively. The first CD4 levels of the patients infected with subtype CRF01_AE (Cluster 1) or CRF01_AE (Cluster 2) were significantly lower than those of CRF07_BC (Z=-4.795, P<0.001; Z=-4.238, P<0.001). Conclusion The genetic subtypes of HIV were mainly CRF01_AE in newly diagnosed HIV-infected patients and this subtype proportion was in increase and the first CD4 levels of the patients were low in Liuzhou during 1998 to 2012. |
View Fulltext
Html FullText
View/Add Comment Download reader |
Close |
|
|
|