山东省某地产超广谱β-内酰胺酶大肠埃希菌耐药情况及多位点序列分型

滕艾颖; 徐留臣; 杨鹏; 孙成云; 陈保立; 王爽; 寇增强; 房明; 王苗苗; 毕振强

Abstract

滕艾颖,徐留臣,杨鹏,孙成云,陈保立,王爽,寇增强,房明,王苗苗,毕振强.山东省某地产超广谱β-内酰胺酶大肠埃希菌耐药情况及多位点序列分型[J].Chinese journal of Epidemiology,2019,40(9):1145-1149

山东省某地产超广谱β-内酰胺酶大肠埃希菌耐药情况及多位点序列分型

Multi locus sequence typing and antibiotic susceptibility of extended-spectrum beta-lactamases producing Enterobacteriaceae in rural residents in villages with pig-breeding farms in Shandong province

Received:January 26, 2019

DOI：10.3760/cma.j.issn.0254-6450.2019.09.024

KeyWord: 大肠埃希菌超广谱β-内酰胺酶多位点序列分型

English Key Word: Escherichia coli Extended-spectrum β-lactamases Multilocus sequence typing

FundProject:国家自然科学基金（81361138021）；山东省自然科学基金（BS2014SW020）

Author Name	Affiliation	E-mail
Teng Aiying	Department of Epidemiology, School of Public Health, Shandong University, Jinan 250012, China
Xu Liuchen	Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
Yang Peng	Zhucheng County Center for Disease Control and Prevention, Zhucheng 262200, China
Sun Chengyun	Zhucheng County Center for Disease Control and Prevention, Zhucheng 262200, China
Chen Baoli	Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
Wang Shuang	Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
Kou Zengqiang	Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
Fang Ming	Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
Wang Miaomiao	Affiliated Hospital of Jining Medical University, Jining 272029, China
Bi Zhenqiang	Department of Epidemiology, School of Public Health, Shandong University, Jinan 250012, China Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China Shandong Key Laboratory for Communicable Disease Control and Prevention, Jinan 250014, China Shandong University Institute of Preventive Medicine, Jinan 250014, China	bzq63@163.com

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Abstract:

目的分析山东省某生猪养殖县农村居民粪便中分离的产超广谱β-内酰胺酶（ESBLs）大肠埃希菌耐药情况和多位点序列分型。方法采用琼脂稀释法对2016年7月收集的山东省某生猪养殖县农村居民新鲜粪便中分离的360株产ESBLs大肠埃希菌进行药物敏感性试验；对CTX-M、TEM、SHV等β-内酰胺酶基因进行PCR扩增，采用毛细管电泳筛选阳性菌株；采用多位点序列分型（MLST）方法进行分子分型，采用eBURST v3.0软件进行聚类分析。结果 360株产ESBLs大肠埃希菌对头孢噻肟、四环素、复方新诺明及氟苯尼考的耐药率较高，分别为100.0%（360/360）、82.2%（296/360）、81.1%（292/360）、80.3%（289/360）。CTX-M基因检出率为99.2%（357/360），以CTX-M-9组、CTX-M-1组为主，分别占35.6%（128/360）和24.4%（88/360）；TEM基因检出率为26.9%（97/360）。MLST共得到132个ST型别，相对优势ST型为ST10，占12.5%（45/360）；聚类分析显示CC10为最主要的克隆群，包括39个ST型，占41.1%（148/360）。结论该农村地区产ESBLs大肠埃希菌对头孢噻肟、四环素、复方新诺明、氟苯尼考的耐药情况较严重，存在以CC10为主的小范围聚集性，可能存在动物与人类之间的传播。

English Abstract:

Objective To analyze the antimicrobial resistance and multilocus sequence typing (MLST) results of extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli in rural residents in villages with pig breeding farms in a county of Shandong province. Methods Antimicrobial susceptibility testing was performed with agar dilution method by using 360 ESBLs-producing E. coli strains from fresh stool samples of rural residents in villages with pig breeding farms in a county of Shandong. PCR was conducted to amplify the CTX-M, TEM, SHV genes and capillary electrophoresis was used to screen positive strains in July, 2016. MLST was performed for molecular typing analysis, and eBURST v3.0 software was used for cluster analysis. Results Among 360 strains of ESBLs-producing E. coli, the resistance rates to cefotaxime, tetracycline, trimethoprim-sulfamethoxazole and florfenicol were 100.0% (360/360), 82.2% (296/360), 81.1% (292/360) and 80.3% (289/360), respectively. The positive rate of CTX-M gene was 99.2% (357/360), in which the positive rate of CTX-M-9 was 35.6% (128/360) and the positive rate of CTX-M-1 was 24.4% (88/360). The positive rate of TEM gene was 26.9% (97/360). A total of 132 STs were identified through MLST. The predominant ST was ST10, accounting for 12.5% (45/360). Cluster analysis showed that CC10 was the most important clone group, including 39 ST clones, involving 148 strains (41.1%). Conclusions The drug resistances of ESBLs-producing E. coli to cefotaxime, tetracycline, trimethoprim-sulfamethoxazole and flurfenicol are serious in this rural area. There is a small-scale clustering of CC10 and transmission mode from animals to humans might exist.

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