| 杜燕华,王若琳,李东晓,马宏,王海峰,许汴利,黄学勇.河南省2018年输入性登革热的病原监测分析[J].Chinese journal of Epidemiology,2020,41(4):542-546 |
| 河南省2018年输入性登革热的病原监测分析 |
| Surveillance on the pathogen of imported Dengue fever in Henan province, 2018 |
| Received:June 14, 2019 |
| DOI：10.3760/cma.j.cn112338-20190614-00437 |
| KeyWord: 登革病毒 病原监测 输入病例 |
| English Key Word: Dengue virus Pathogen surveillance Imported case |
| FundProject:河南省科技攻关项目（172102310338）；河南省医学科技攻关计划普通项目（201602315） |
| Author Name | Affiliation | E-mail | | Du Yanhua | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Wang Ruolin | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Li Dongxiao | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Ma Hong | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Wang Haifeng | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Xu Bianli | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | | | Huang Xueyong | Henan Provincial Key Laboratory of Infectious Pathogenic Microorganisms, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China | Hyxzzu@163.com |
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| Abstract: |
| 目的 分析2018年河南省输入性登革热的病原监测情况。方法 利用国家传染病报告信息管理系统,收集2018年河南省登革热疑似病例,开展个案调查同时采集血清,检测登革病毒NS1抗原、IgM和IgG抗体以及病毒RNA;对于病毒RNA检测阳性的样本进行荧光PCR分型诊断和E基因序列扩增,阳性扩增产物测序后进行同源性比对和系统进化分析。结果 2018年河南省累计报告登革热病例29例,均为输入性病例,来自东南亚地区(25/27,92.6%)和非洲地区(2/27,7.4%),以<45岁中青年农民为主,男性明显多于女性,输入性病例时间空间分布分散。29例报告病例中NS1抗原和/或IgM检测阳性的22例。8例病例标本进行核酸检测,其中6例阳性且基因分型成功,其中登革病毒1型、2型各3例。1例由马尔代夫输入的2型登革病毒进行了测序和系统进化分析,与2008年柬埔寨2型登革病毒JF730046一致性最高,属于AsianⅠ基因亚型。结论 2018年河南省输入性登革热病例较2017年明显上升,但未引起河南省本地流行。 |
| English Abstract: |
| Objective To analyze data gathered from the laboratory records related to imported Dengue cases in Henan province in 2018. Methods Suspected Dengue cases were found out through the Dengue fever surveillance network from the National infectious Disease reporting management information system. Serum samples of suspected Dengue cases were collected while case study and tested for Dengue NS1 antigen, IgM, IgG antibodies and Dengue RNA in Henan province in 2018. According to the standardized Dengue diagnosis criteria, confirmed cases were identified under the results of testing. Dengue RNA was checked by Real-time PCR genotyping and amplification of E gene in the samples being tested, before the PCR products were sequenced and analyses of homological and phylogenetic were performed. Results In 2018, a total of 29 cases of Dengue fever was reported in Henan province, with all of which were imported cases, mainly from Southeast Asian countries and Africa. Majority of the cases were young and middle-aged farmers under 45 years old, and the number of males was significantly higher than that of females. The imported cases were dispersed in time and space. Among the 29 Dengue reported cases, 22 cases were with NS1 antigen and/or IgM positive through testing, while 6 cases were positive by detection of the Dengue virus RNA. These 6 samples with Dengue RNA were genotyped successfully, including 3 cases of Dengue virus type 1 and 3 cases of type 2. One of the Maldives import Dengue virus type 2 samples was sequenced. Result showed that the sequence belonged to the Asian Ⅰ genotype, which was most consistently similar to the Cambodia's Dengue virus type 2 JF730046, identified in 2008. Conclusions The incidence of imported Dengue fever cases increased significantly in Henan province in 2018, compared to that in 2017, but fortunately did not cause any local epidemics. |
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