| 程睿,南晓伟,范娜,付士红,司晓艳,张琳,何英,雷雯雯,李樊,王环宇,鲁晓晴,梁国栋.内蒙古自治区采集的蚊虫标本新发现乙脑病毒和盖塔病毒[J].Chinese journal of Epidemiology,2020,41(4):571-579 |
| 内蒙古自治区采集的蚊虫标本新发现乙脑病毒和盖塔病毒 |
| Emerging of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia Autonomous Region |
| Received:April 25, 2019 |
| DOI：10.3760/cma.j.cn112338-20190425-00284 |
| KeyWord: 乙脑病毒 盖塔病毒 虫媒病毒 |
| English Key Word: Japanese encephalitis Getah virus Arbovirus |
| FundProject:生物安全关键技术研发重点专项（2016YFC1201904） |
| Author Name | Affiliation | E-mail | | Cheng Rui | School of Public Health, Qingdao University, Qingdao 266071, China
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Nan Xiaowei | Department of Vector Biological Prevention and Control, Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, China | | | Fan Na | School of Public Health, Qingdao University, Qingdao 266071, China
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Fu Shihong | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Si Xiaoyan | Department of Vector Biological Prevention and Control, Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, China | | | Zhang Lin | Bayannaoer Center for Disease Control and Prevention, Bayannaoer 015000, China | | | He Ying | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Lei Wenwen | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Li Fan | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Wang Huanyu | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Lu Xiaoqing | School of Public Health, Qingdao University, Qingdao 266071, China | Luxq532@126.com | | Liang Guodong | Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | gdliang@hotmail.com |
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| Abstract: |
| 目的 了解内蒙古自治区的吸血昆虫及其携带虫媒病毒的种类及分布,为虫媒病毒病的预防提供基础数据。方法 在自然界使用灯诱法采集蚊虫标本,形态学分类后分装编号,液氮保存。组织培养法进行病毒分离。使用多种生物学信息软件(BioEdit、MEGA等)对病毒核苷酸序列进行分子生物学特征分析。结果 2014、2017-2018年在内蒙古自治区5个县(旗)的采集点共采集到2属3种蚊虫24 240只,蠓虫17 110只。其中在淡色库蚊标本中检测到乙脑病毒基因阳性,并且获得4株可以稳定传代的病毒分离物,经分子生物学鉴定分别为盖塔病毒、浓核病毒。盖塔病毒(NMDK1813-1)E2基因遗传进化分析结果显示,与甘肃分离株(GS10-2)在同一进化分支,且有6个共同的氨基酸变异位点。结论 本研究发现的乙脑病毒和盖塔病毒均是在内蒙古蚊虫标本中新近发现的病毒,这为内蒙古地区的虫媒病毒及其传播疾病的预防及控制提供了基础数据,对内蒙古的虫媒病毒及其病毒病的预防及控制提出了新的挑战。 |
| English Abstract: |
| Objective To investigate the types and distribution of blood-sucking insects and arboviruses in Inner Mongolia autonomous region, and provide basic data for the prevention of arbovirus transmitted disease. Methods Blood-sucking insects were collected by lamp trapping method in nature. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. Viruses were isolated in cell culture and characterized, using molecular biological methods. Results A total of 24 240 mosquitoes and 17 110 aphids were collected from 2 sites of 5 counties (Flags) in Inner Mongolia in 2014 and during 2017-2018. Among them, Japanese encephalitis virus gene was detected in Culex pipiens pallens, and 4 virus strains isolates which could be stably passaged. The isolates were identified as Getah virus and densonucleosis virus by molecular biology identification. Phylogenetic analysis on the E2 gene of the Getah virus (NMDK1813-1) showed that it belonged to the same evolutionary branch of the Gansu isolates (GS10-2) and having six common amino acid variation sites. Conclusions The emergence of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia indicated the new challenges on the prevention and control of arbovirus and related diseases. The results pf this study provided basic data for the prevention and control stretagies of arbovirus transmitted diseases in Inner Mongolia. |
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