煤矿工人外周血端粒长度和线粒体DNA拷贝数与高血压的关系研究

高媛媛; 郭建勇; 张真; 韩致超; 雷立健; 孙晨明; 黄建军; 王彤

Abstract

高媛媛,郭建勇,张真,韩致超,雷立健,孙晨明,黄建军,王彤.煤矿工人外周血端粒长度和线粒体DNA拷贝数与高血压的关系研究[J].Chinese journal of Epidemiology,2020,41(5):727-732

煤矿工人外周血端粒长度和线粒体DNA拷贝数与高血压的关系研究

Relationship of telomere length, mitochondrial DNA copy number of peripheral blood with hypertension in coal miners

Received:September 30, 2019

DOI：10.3760/cma.j.cn112338-20190930-00714

KeyWord: 高血压端粒长度线粒体DNA拷贝数交互作用

English Key Word: Hypertension Telomere length Mitochondrial DNA copy number Interaction

FundProject:国家自然科学基金（81872701）；山西省研究生联合培养基地人才培养项目（2016JD24）

Author Name	Affiliation	E-mail
Gao Yuanyuan	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Guo Jianyong	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Zhang Zhen	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Han Zhichao	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Lei Lijian	School of Public Health, Shanxi Medical University, Taiyuan 030001, China	wwdlijian@sxmu.edu.cn
Sun Chenming	General Hospital of Datong Coal Mine Group, Datong 037000, China
Huang Jianjun	General Hospital of Datong Coal Mine Group, Datong 037000, China
Wang Tong	School of Public Health, Shanxi Medical University, Taiyuan 030001, China

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Abstract:

目的分析煤矿工人中高血压与外周血端粒长度、线粒体DNA拷贝数（mtDNA-CN）的关系以及端粒长度与mtDNA-CN对高血压的交互作用。方法 2013年7-12月在山西省某煤矿集团开展病例对照调查研究，选取325名健康工人作为对照组，378名高血压工人作为病例组。通过问卷调查收集研究对象的一般人口学特征、生活行为习惯等信息；采用实时定量PCR检测外周血端粒长度、mtDNA-CN水平；运用logistic回归分析高血压与端粒长度、mtDNA-CN及其他因素的关系，采用相加、相乘模型分析端粒长度与mtDNA-CN对高血压的交互作用。结果病例组工人的平均端粒长度为（1.50±0.55）kb，对照组工人的平均端粒长度为（2.01±0.62）kb，两组间的差异有统计学意义（t=11.68，P<0.001）；相关分析结果显示，在病例组，端粒长度与mtDNA-CN呈正相关（r=0.157，P=0.002）；多因素分析结果显示，端粒长度（OR=4.408，95% CI：3.012~6.452）、年龄（OR=0.417，95% CI：0.284~0.613）、BMI（OR=1.357，95% CI：1.162~1.584）、家庭月均收入（OR=0.656，95% CI：0.553~0.778）和工龄（OR=1.249，95% CI：1.100~1.417）均是高血压的影响因素；且端粒长度与mtDNA-CN对高血压有相乘交互作用（OR=1.267，95% CI：1.094~1.468）。结论端粒长度较短是高血压的危险因素，端粒长度与mtDNA-CN对高血压的相乘交互作用存在，但未发现mtDNA-CN与高血压相关。

English Abstract:

Objective To explore the relationship of telomere length, mitochondrial DNA copy number of peripheral blood with hypertension and the interaction between telomere length and mtDNA-CN on hypertension in coal miners. Methods A case control study was conducted in a coal mine of Shanxi province from July to December of 2013, in which 325 healthy workers were selected as the control group and 378 workers with hypertension as the case group. The information about general demographic characteristics and life behavior habits of the subjects were collected through questionnaire. Levels of telomere length and mtDNA-CN in peripheral blood were detected by real-time PCR. Unconditional logistic regression was used to examine the association between hypertension and telomere length, mtDNA-CN. The interaction test between telomere length and mtDNA-CN on hypertension was performed by adding the interaction term in the corresponding model. Results The mean telomere length of the workers in the case group was (1.50±0.55) kb, and that of the control group was (2.01±0.62) kb, the difference between two groups was significant (t=11.68, P<0.001). The correlation analysis showed that telomere length was positively correlated with mtDNA-CN (r=0.157, P=0.002) in the case group. Multivariate analysis showed that telomere length (OR=4.408, 95%CI:3.012-6.452), age (OR=0.417, 95%CI:0.284-0.613), BMI (OR=1.357, 95%CI:1.162-1.584), monthly household income level (OR=0.656, 95%CI:0.553-0.778) and work duration (OR=1.249, 95%CI:1.100-1.417) were influencing factors of hypertension. The multiply interaction between telomere length and mtDNA-CN was significant on hypertension (OR=1.267, 95%CI:1.094-1.468). Conclusions The results suggest shorter telomere length is a risk factor of hypertension. There is a multiply interaction between telomere length and mtDNA-CN on hypertension. However, the association between mtDNA-CN and hypertension was not found.

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