| 武佳欣,杨志清,张睿君,李雁笛,赵甜静,扆琳珠,冯永亮,丰淑英,汪波,王素萍.HBsAg阳性母亲HBV核心启动子突变与宫内传播的关系[J].Chinese journal of Epidemiology,2020,41(6):902-907 |
| HBsAg阳性母亲HBV核心启动子突变与宫内传播的关系 |
| Relationship between mutations of HBV basal core promoter region in HBsAg-positive mothers and intrauterine transmission |
| Received:February 24, 2020 |
| DOI：10.3760/cma.j.cn112338-20200224-00163 |
| KeyWord: 乙型肝炎病毒宫内传播 核心启动子 HBs抗原 HBe抗原 A1762T/G1764A |
| English Key Word: Intrauterine transmission Basal core promoter HBsAg HBeAg A1762T/G1764A |
| FundProject:国家自然科学基金（81573212，81872677）；传染病预防控制国家重点实验室自主研究课题（2017SKLID306，2018SKLID310） |
| Author Name | Affiliation | E-mail | | Wu Jiaxin | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Yang Zhiqing | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Zhang Ruijun | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Li Yandi | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Zhao Tianjing | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Yi Linzhu | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | | | Feng Yongliang | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | fengyongliang048@163.com | | Feng Shuying | Department of Obstetrics and Gynaecology, the Third People Hospital of Taiyuan City, Taiyuan 030001, China | | | Wang Bo | Department of Obstetrics and Gynaecology, the Third People Hospital of Taiyuan City, Taiyuan 030001, China | | | Wang Suping | Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China | spwang88@163.com |
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| Abstract: |
| 目的 分析HBV为C基因型的HBsAg阳性母亲核心启动子（BCP）区突变与宫内传播的关系。方法 2011年6月至2013年7月太原市第三人民医院妇产科HBsAg阳性母亲及新生儿399对。收集一般人口学资料，采用荧光定量PCR和电化学发光法分别检测母婴血清HBV DNA及HBV血清学标志物。选择HBV DNA载量≥106 IU/ml的113例母亲为研究对象，其新生儿发生宫内传播的22例为宫内传播组，随机选取其中22例未发生宫内传播者作为对照组，母亲HBV DNA经提取、扩增、克隆、测序和序列编辑及剪接后与从NCBI下载的标准序列比对进行基因分型，最终选择C基因型的39例母亲进行突变分析。结果 HBV为C基因型（88.63%）的母亲共39例，其中宫内传播组19例，对照组20例。母亲A1762T/G1764A双突变率在两组差异显著（7.53% vs.27.72%，P<0.001）。非条件logistic回归分析显示A1762T/G1764A双突变可能是宫内传播的保护因素（aOR=0.065，95% CI：0.006～0.746，P=0.028）。母亲A1762T/G1764A双突变可能与新生儿HBeAg水平有关（P=0.050）。结论 HBV C基因型的HBsAg阳性母亲HBV DNA BCP区A1762T/G1764A双突变可能降低HBV宫内传播的风险。 |
| English Abstract: |
| Objective To analyze the relationship between maternal mutations in basal core promoter region of hepatitis B virus (HBV) genotype C and intrauterine transmission. Methods We collected information on general demographic characteristics and process of delivery among 399 pairs of consecutive HBsAg-positive mothers and their neonates, from the Third People’s Hospital of Taiyuan in Shanxi province, China. Fluorescence quantitative polymerase chain reaction (FQ-PCR) and Electro-chemiluminescence immuno-assay (ECLIA) kits were used to detect both maternal and neonatal HBV DNA and serological markers in the peripheral blood. From 113 mothers with HBV DNA load ≥106 IU/ml, we selected 22 mothers whose neonates were with intrauterine transmission and randomly selected the same number of mothers whose neonates were without intrauterine transmission, as controls. The whole-length HBV DNA were extracted, amplified, cloned, sequenced and genotyped. Finally, a total of 39 mothers with genotype C of HBV were selected for mutation analysis. Results Thirty-nine cases of genotype C (88.63%) were finally included in the study, with 19 cases in the intrauterine transmission group and 20 cases as controls. Rates of A1762T/G1764A double mutations were significantly different between the intrauterine transmission group and the control group (7.53% vs. 27.72%, P<0.001). Results from the multivariate analysis showed that the A1762T/G1764A double mutations had reduced the risk of intrauterine transmission (aOR=0.065, 95%CI: 0.006-0.746, P=0.028). Maternal A1762T/G1764A double mutations appeared to be possibly associated with neonatal HBeAg (P=0.050). Conclusion A1762T/G1764A double mutations of HBV DNA from the genotype C of those HBsAg-positive mothers could reduced the risk of HBV intrauterine transmission during pregnancy. |
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