| 蔡亮,张恒娇,何方玲,奉雅乐,胡世雄,王娟,刘富强,蒋永林,谭夏林,潘海明,唐宾彬,杨浩,龙浩宇,湛志飞,高立冬.湖南省2018年登革热本地暴发流行病学和病毒分子特征分析[J].Chinese journal of Epidemiology,2020,41(12):2119-2124 |
| 湖南省2018年登革热本地暴发流行病学和病毒分子特征分析 |
| Epidemiological and virus molecular characterization of dengue fever outbreak in Hunan province, 2018 |
| Received:January 07, 2020 |
| DOI：10.3760/cma.j.cn112338-20200107-00018 |
| KeyWord: 登革热 暴发流行 病原学 分子特征 |
| English Key Word: Dengue fever Outbreak Pathogen Molecular characteristics |
| FundProject:国家科技重大专项（2018ZX10713002001003）；湖南省卫生健康委员会重点课题（A2011-006） |
| Author Name | Affiliation | E-mail | | Cai Liang | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Zhang Hengjiao | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | He Fangling | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Feng Yale | Qiyang Country Center for Disease Control and Prevention of Yongzhou, Qiyang 426100, China | | | Hu Shixiong | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Wang Juan | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Liu Fuqiang | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Jiang Yonglin | Yongzhou Prefectural Center for Disease Control and Prevention, Yongzhou 425000, China | | | Tan Xialin | Yongzhou Prefectural Center for Disease Control and Prevention, Yongzhou 425000, China | | | Pan Haiming | Qiyang Country Center for Disease Control and Prevention of Yongzhou, Qiyang 426100, China | | | Tang Binbin | Qiyang Country Center for Disease Control and Prevention of Yongzhou, Qiyang 426100, China | | | Yang Hao | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Long Haoyu | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Zhan Zhifei | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | | | Gao Lidong | Hunan Provincial Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China | gldlj@hotmail.com |
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| Abstract: |
| 目的 对湖南省2018年登革热本地暴发疫情进行流行病学及病原学特征研究。方法 对报告的8例疑似登革热病例进行实验室诊断，对病例密切接触者搜索出的186例疑似登革热病例和发热病例开展病原学监测，应用C6/36细胞对病例急性期血清开展病毒分离，对15株登革病毒株E基因测序，分析病毒的血清型别和基因亚型，构建系统发生树，分析可能的传播来源。在疫点开展蚊媒密度应急监测和健康人群回顾性血清流行病学调查。结果 8例疑似病例血清标本，6例登革病毒核酸阳性，4例登革病毒NS1抗原阳性。186例疑似登革热病例，96例病原学检测结果阳性，分离到登革病毒株64株，经鉴定全部为登革病毒2型全球型，来源于广东和浙江省的可能性较大。应急蚊媒密度监测，疫点布雷图指数最高达65，具有极高的登革热传播风险。回顾性调查377名健康人群进行登革热抗体水平监测，IgG抗体阳性率为0.53%（2/377）。结论 现场流行病学调查和分子遗传分析提示，湖南省2018年本地暴发疫情由输入性病例引起，由单一的登革病毒2型全球型引起。 |
| English Abstract: |
| Objective To analyze the epidemiological and etiological characteristics of a dengue fever outbreak in Hunan province in 2018. Methods Real-time PCR assay was performed for the laboratory diagnosis of 8 suspected dengue fever cases. Etiological surveillance was performed in 186 suspected dengue fever cases and fever cases who had close contacts with dengue fever patients. C6/36 cells was used for the virus isolation from acute phase serum. By sequencing the full length of E genes of 15 dengue virus strains, phylogenetic analysis was performed based on the sequences obtained, including reference sequences from the NCBI GenBank database, the serotypes and gene subtypes of the virus were analyzed to trace the possible source of transmission. An emergency monitoring of vector density and a retrospective survey of sero-epidemiology in healthy population were conducted in the epidemic area. Results In the serum samples of 8 suspected patients, 6 were dengue virus RNA positive, and 4 were NS1 antigen positive. In 186 suspected patients, 96 were dengue virus nucleic acid, NS1 antigen or antibody positive in etiological test. A total of 64 dengue virus strains were isolated. The phylogenetic analysis showed that all the dengue virus strains belonged to type 2, which might be from Guangdong or Zhejiang provinces. The Bretub index was up to 65, indicating an extremely high risk of transmission. The positive rate of the dengue virus IgG antibody was 0.53%(2/377) in retrospective survey of 377 healthy people. Conclusion The field epidemiologic and the molecular genetics analyses showed the outbreak of dengue fever in Hunan in 2018 was caused by imported cases and dengue virus 2. |
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