| 胡豫杰,许学斌,王艳,王伟,崔鑫楠,何琼玉,李孟寒,徐进,李凤琴.侵袭性非伤寒沙门菌血清型和耐药性分析[J].Chinese journal of Epidemiology,2021,42(3):549-554 |
| 侵袭性非伤寒沙门菌血清型和耐药性分析 |
| Analysis on serotype and antimicrobial resistance of invasive non-typhoidal Salmonella |
| Received:March 16, 2020 |
| DOI：10.3760/cma.j.cn112338-20200316-00361 |
| KeyWord: 侵袭性非伤寒沙门菌 血清型 耐药性 |
| English Key Word: Invasive non-typhoidal Salmonella Serotype Antimicrobial resistance |
| FundProject:国家重点研发计划（2018YFC1603904）；国家科技重大专项（2016ZX08011005） |
| Author Name | Affiliation | E-mail | | Hu Yujie | Key Laboratory of Food Safety Risk Assessment of Health, National Health Commission of China, China National Center for Food Safety Risk Assessment, Beijing 100021, China | | | Xu Xuebin | Institute of Pathogen Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China | | | Wang Yan | Department of Clinical Laboratory, Beijing Jishuitan Hospital, Beijing 100035, China | | | Wang Wei | Key Laboratory of Food Safety Risk Assessment of Health, National Health Commission of China, China National Center for Food Safety Risk Assessment, Beijing 100021, China | | | Cui Xinnan | Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China | | | He Qiongyu | Centre for Food Safety, University College Dublin, Dublin D04 N2E5, Ireland | | | Li Menghan | Key Laboratory of Food Safety Risk Assessment of Health, National Health Commission of China, China National Center for Food Safety Risk Assessment, Beijing 100021, China | | | Xu Jin | Key Laboratory of Food Safety Risk Assessment of Health, National Health Commission of China, China National Center for Food Safety Risk Assessment, Beijing 100021, China | | | Li Fengqin | Key Laboratory of Food Safety Risk Assessment of Health, National Health Commission of China, China National Center for Food Safety Risk Assessment, Beijing 100021, China | lifengqin@cfsa.net.cn |
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| Abstract: |
| 目的 分析7株侵袭性非伤寒沙门菌（iNTS）的血清型和耐药性。方法 针对收集的7株iNTS菌株，开展血清学鉴定、药物敏感性实验以及全基因组测序，并对血清型、MLST型和耐药基因进行鉴定、注释和分析。结果 7株iNTS菌株中，包含1株鼠伤寒血清型和2株Ⅰ 4，［5］，12：i：-（ST34型）、2株肠炎血清型、1株科瓦利斯血清型和1株未知血清型Ⅰ 4，［5］，12：d：-（ST279型），其中6株为单相菌，沙门菌二相鞭毛基因缺失或假基因化可能有助于增强沙门菌侵袭性。未发现替加环素、氨曲南、阿米卡星及头孢类、碳青霉烯类抗生素耐药株，存在1株八重耐药鼠伤寒沙门菌。耐药基因与耐药表型基本相符。结论 本研究中的iNTS菌株存在八重耐药株，整体耐药水平尚低但不可忽视。 |
| English Abstract: |
| Objective To investigate the serotypes and antimicrobial resistance of seven invasive non-typhoidal Salmonella (iNTS) isolates. Methods For 7 iNTS strains collected, serotype identification, antimicrobial susceptibility testing and whole genome sequencing were performed. We identified, annotated and analyzed the serotypes, MLST types, and antimicrobial resistance genes. Results Among the 7 tested iNTS isolates, we found one Salmonella Typhimurium strain and two Salmonella Ⅰ 4,[5], 12:i:-strains whose MLST types were ST34, two Salmonella Enteritidis strains, one Salmonella Corvallis strain and one strain of unknown serotype with the antigenic formulae of Ⅰ 4,[5], 12:d:-(ST279 type). Six of seven strains were monophasic and the deletion or pseudogenization of Salmonella Flagellum gene might contribute to the enhancement of Salmonella invasiveness. None was found to be resistant to tigarcycline, aztreonam, amikacin, cephalosporins and carbapenem and one Salmonella Typhimurium strain was found to be co-resistant to eight classes of antimicrobials at the same time. Resistance genes were generally in accord with relative resistant phenotypes. Conclusion The iNTS strains could show high level multi-drug resistance, indicating that close attention should be paid to the resistance of iNTS though the overall resistance might be relatively not high. |
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