| 王爽,徐留臣,裴耀文,王梅,毕振强,张华宁,刘璐,房明,寇增强.农村井水中耐碳青霉烯类铜绿假单胞菌及耐药基因分子流行病学特征[J].Chinese journal of Epidemiology,2021,42(5):898-902 |
| 农村井水中耐碳青霉烯类铜绿假单胞菌及耐药基因分子流行病学特征 |
| Molecular epidemiology of drug resistance genes and carbapenem resistance of Pseudomonas aeruginosa in rural well water |
| Received:September 04, 2020 |
| DOI：10.3760/cma.j.cn112338-20200904-01127 |
| KeyWord: 铜绿假单胞菌 碳青霉烯类 耐药基因 分子流行病学 |
| English Key Word: Pseudomonas aeruginosa Carbapenem Drug resistance genes Molecular epidemiology |
| FundProject:国家科技重大专项（2018ZX10714002-003-004）；山东省重点研发计划（2017GSF218062）；山东省医药卫生科技发展计划（2017WS105） |
| Author Name | Affiliation | E-mail | | Wang Shuang | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Xu Liuchen | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Pei Yaowen | General Office, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Wang Mei | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Bi Zhenqiang | General Office, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Zhang Huaning | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Liu Lu | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Fang Ming | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | | | Kou Zengqiang | Insitute of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Ji'nan 250014, China | jack-cou@163.com |
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| Abstract: |
| 目的 分析农村井水中耐碳青霉烯类铜绿假单胞菌（PA）及耐药基因的分子流行病学特征。方法 依据《饮用天然矿泉水检验方法》（GB 8538-2016）对山东省巨野县农村井水采集112份水样进行检测，并对检出的PA分别进行PFGE分型和药物敏感性试验。PCR鉴定碳青霉烯类耐药基因后，采用S1-PFGE和Southern杂交确定耐药基因的位置，并用结合实验判断基因的可转移性。结果 农村井水中PA检出率为54.46％（61/112）。61株菌分为56个PFGE型，100.00%一致带型最多的有2株菌，无明显的优势带型。药敏实验结果显示，多重耐药菌占93.44％（57/61），2株菌携带blaVIM-2耐药基因，均位于质粒上，且均可随质粒发生水平转移。结论 农村井水中检出耐碳青霉烯类PA及其耐药基因，此类耐药基因存在水平传播的可能性。 |
| English Abstract: |
| Objective To analyze molecular epidemiological characteristics of drug resistance genes and carbapenem resistance of Pseudomonas aeruginosa (PA) in rural well water. Methods According to Citation of Natural Mineral Water Inspection (GB 8538-2016), a total of 112 well water samples were tested in Juye county of Shandong province, and PFGE and drug susceptibility test were conducted for the identified PA strains. After PCR identification of carbapenem resistance genes, S1-PFGE and Southern blotting were used to determine the location of drug resistance genes, and combined experiments were used to determine gene transferability. Results The detection rate of PA in rural well water samples in Juye county of Shandong province was 54.46% (61/112). The 61 strains could be divided into 56 PFGE types. There were 2 strains with 100.00% consistent band types, and there was no obvious predominant band type. The results of drug susceptibility experiments showed that 93.44% (57/61) were multi-drug resistant strains, and there were 2 strains carrying blaVIM-2, both of which were located on the plasmid, and both of them were transferred horizontally with the plasmid.Conclusion PA carrying carbapenem resistance genes was detected in well water of rural communities in Juye country, and there is the possibility of horizontal transmission of such resistance genes. |
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