张楠,张爽,王锋,尹遵栋,苏秋东,张国民,缪宁,毕胜利,王富珍,沈立萍.我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析[J].Chinese journal of Epidemiology,2022,43(6):865-870 |
我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析 |
Epidemiological distribution of hepatitis B virus genotypes in acute hepatitis B cases in China,2015-2017 |
Received:December 30, 2021 |
DOI:10.3760/cma.j.cn112338-20211230-01041 |
KeyWord: 急性乙型肝炎 乙型肝炎病毒 基因型 分子流行病学 |
English Key Word: Acute Hepatitis B Hepatitis B virus Genotype Molecular epidemiology |
FundProject:国家科技重大专项(2017ZX10105015-001-002) |
Author Name | Affiliation | E-mail | Zhang Nan | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Zhang Shuang | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Wang Feng | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Yin Zundong | Department of National Immunization Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | Su Qiudong | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Zhang Guomin | Department of National Immunization Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | Miao Ning | Department of National Immunization Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | Bi Shengli | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | Wang Fuzhen | Department of National Immunization Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China | wangfz@chinacdc.cn | Shen Liping | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | shenlp@ivdc.chinacdc.cn |
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Abstract: |
目的 分析我国急性乙型肝炎(乙肝)病例HBV基因型流行病学分布特征。方法 收集2015-2017年中国疾病预防控制信息系统报告的急性乙肝病例620例,通过巢式PCR扩增获得全长HBV基因组,构建系统发育树确定HBV基因型,结合基础资料分析HBV基因型的分布情况。结果 在620例急性乙肝病例中,成功分型519例(83.71%,519/620),包括A型(0.19%,1/519)、B型(27.17%,141/519)、C型(62.04%,322/519)、D型(9.06%,47/519)、I型(0.77%,4/519)和CD重组型(0.77%,4/519);2个主要的基因亚型为B2(95.03%,134/141)和C2(72.67%,234/322)。基因型在我国7个地区分布不同,C型在东北(94.55%,52/55)、华北(93.85%,61/65)、华东(78.87%,56/71)和华南地区(58.14%,50/86)的比例较高,B型在华中(58.07%,36/62)和西南(52.94%,45/85)地区的比例较高,西北地区的D型(48.42%,46/95)比例较高;共有515例成功获得血清型,包括adr(57.48%,296/515)、adw(30.87%,159/515)、ayr(0.19%,1/515)、ayw(11.46%,59/515)。B型以adw血清型(92.14%,129/140)为主,C型以adr血清型(91.88%,294/320)为主,D型均为ayw血清型;不同性别、年龄组中,基因型分布差异无统计学意义(P>0.05)。结论 2015-2017年我国急性乙肝基因型以B、C和D型为主,东北、华北、华东和华南地区C型为主;华中、西南地区B、C型均有,B型为主,D型多分布于西北地区;急性乙肝基因型与血清型存在相关性,B型以adw血清型为主,C型以adr血清型为主,D型均为ayw血清型;不同性别和年龄组中急性乙肝基因型分布无差异。 |
English Abstract: |
Objective To analyze the genotype distribution of acute hepatitis B virus in China. Methods A total of six hundred and twenty acute Hepatitis B cases reported to China Information System for Diseases Control and Prevention from 2015 to 2017 were selected. First, the full-length HBV genome was obtained by nested PCR amplification. In addition, the HBV genotype was determined by constructing a phylogeny tree. Finally, using primarydata, HBV genotype distribution was analyzed. Results A total of 519 (83.71%, 519/620) sequences were obtained genotype of 620 acute hepatitis B cases, including A (0.19%, 1/519), B (27.17%, 141/519), C (62.04%, 322/519), D (9.06%, 47/519), I (0.77%, 4/519) and C/D (0.77%, 4/519); B2(95.03%, 134/141) and C2 (72.67%, 234/322) were the two major subgenotypes. Genotypes were distributed differently in seven regions of China. The proportion of genotype C appeared higher in Northeast China (94.55%, 52/55), North China (93.85%, 61/65), East China (78.87%, 56/71), and South China (58.14%, 50/86). The proportion of genotype B was higher in Central China (58.07%, 36/62) and Southwest China (52.94%, 45/85), the proportion of genotype D was the highest in Northwest China (48.42%, 46/95). A total of 515 cases were classified as serotypes, including 'adr' (57.48%, 296/515), 'adw' (30.87%, 159/515), 'ayr' (0.19%, 1/515), and 'ayw' (11.46%, 59/515). Genotype B was dominated by 'adw' serotype (92.14%, 129/140), genotype C was dominated by 'adr' serotype (91.88%, 294/320),all genotype D were 'ayw' serotype. The genotype of acute hepatitis B was correlated with serotype, 'adw' was dominant in genotype B, 'adr' was dominant in genotype C and 'ayw' was dominant in genotype D.In different gender and age group, there was no statistical significance ingenotype distribution (P>0.05). Conclusions The genotype of acute hepatitis B in China from 2015 to 2017 was mainly B, C, and D; genotype C was dominant in the Northeast China,North China, East China and South China; B and C were common in Central and Southwest China, and genotype B was dominant. Genotype D was primarily distributed in Northwest China. The genotype of acute hepatitis B was correlated with serotype, 'adw' was dominant in genotype B, 'adr' was dominant in genotype C and 'ayw' was dominant in genotype D. There was no difference in the distribution of acute hepatitis B genotypes among different genders and age groups. |
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