| 过祥豹,李良寿,汪美先.对炭疽阿斯可利试验效果的检验[J].Chinese journal of Epidemiology,1985,6(1):50-51 |
| 对炭疽阿斯可利试验效果的检验 |
| Evaluation of the diagnostic valve of Ascoli Test in Anthrax |
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| Abstract: |
| 检查炭疽皮毛108份,培养阳性率为62%,阿斯可利试验阳性率仅24.1%;正常组织81份,阿氏试验有28.4%的假阳性;炭疽组织标本140份,阿氏试验和培养结果总符合率仅57.9%,两者关系不密切。说明阿氏试验的敏感性不高,特异性不强。特异性不强因炭疽沉淀素血清中含有类属抗体,与蜡样杆菌等发生交叉反应,並经免疫荧光法证明且吸收试验难以去除。敏感性不高因环状沉淀法是血清学中敏感性最低者,难以检出微量抗原。故判断阿氏试验结果应十分慎重,在教学、科研和检验工作中如何正确对待阿氏试验,本文提出了一些看法。 |
| English Abstract: |
| 108 samples of diseased animal skin & fur were tested, it was found that 62% were positive for Bacillus anthracis by cultivation, but only 24.1% positive by Ascoli test.There were 28.4% false positive results with normal animal tissues by Ascoli test. In 140 samples of anthrax animal tissues, it was found that 57.98% correlation was found between Ascoli test and bacterial cultivation and such relationship was not close. These results revealed that Ascoli test possessed low sensitivity and specificity. The cause of low specificity of Ascoli test was that there were common antibodies in the diagnostic precipitin serum, they might react with antigens of Bacillus cereus thus eliciting cross reactions. This was also proven by fluorescent antibody technic and the cros reacting autibodies could not be removed by absorption test. The background of low sensitivity by ring precipitation method seems to be that the sensitivity of precipitin test in serology being the lowest of all, it could not detect out minute amount of antigen. So interpretation of the results of Ascoli test should be very careful. Our view point about the value of Asco li test used in research, diagnosis, and instruction in Microbiology is pointed out. |
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