| 吴惠联,郭进林,韩文清,张玉昆,孟红,冯连珠.ELISA检测正常人血清轮状病毒抗体的研究[J].Chinese journal of Epidemiology,1986,7(4):203-205 |
| ELISA检测正常人血清轮状病毒抗体的研究 |
| A Study on the Rotavirus Antibody in the Normal Human Sera by Using ELISA |
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| Abstract: |
| 用ELISA间接法检测277份不同年龄组正常人血清标本的轮状病毒抗体,6个月~1岁组抗体阳性率最低,为51.5%,6个月以下组为53.0%,1岁以上各小儿组由83.8%渐增至92.3%,成人组及脐带血均100%。对其中82份标本同时作ELISA与补体结合法检测,前者阳性率92.68%,抗体GMT为894.6;后者阳性率79.27%,GMT为8.136,总符合率为90.24%,两法有良好的正相关性,相关系数r=0.67,ELISA比CFT具更高的敏感性。 |
| English Abstract: |
| In Jinan area, 277 serum samples from healthy persons of different ages were tested by ELISA for the presence of Rotarirus (RV) antibody. The positive rate gradually increases (83.8-100%) with age from 1 year to adulthood. The rate is the lowest (51.5%) in the 6 months-1 year age group and 53.0%in the group of less than 6 months. Eighty-two of the 277 samples were determined both by ELISA and complement fixation test (CFT). In ELISA the positivity of RV antibody was 92.68% and its GMT was 894.6. In CFT the positivity was 79.27% and GMT was 8.136. The concordance rate of the two methods was 90.24%. The correlation coefficient (r) was +0.67. However, the titer of RV antibody in the sera determined by ELISA was 110 times higher than that by CFT. It means that ELISA is more sensitive than CFT. The result of blocking test further confirmed the specificity of ELISA method. Therefore, it is a simple rapid and reliable method to be used in clinical diagnosis and epidimiologic investigation of RV infection. |
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