| 吕德生,陈志新,王伯伦.空肠/结肠弯曲菌的生物分型和质粒分析及其在流行病学中的初步应用[J].Chinese journal of Epidemiology,1988,9(1):38-41 |
| 空肠/结肠弯曲菌的生物分型和质粒分析及其在流行病学中的初步应用 |
| Biotyping and Plasmid Analysis of Campylobacter jejuni/coli and Their Preliminary Application in Epidemiological Study |
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| DOI： |
| KeyWord: 空肠/结肠弯曲菌 生物分型 质粒分析 |
| English Key Word: Campylobacter jejuni/coli Biotyping Plasmid analysis |
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| Abstract: |
| 本文对来自成都地区的弯曲菌进行了生物分型和质粒分析,并结合血清学分型应用于弯曲菌肠炎病人家庭的调查。116株弯曲菌中,空肠弯曲菌生物1型、生物2型和结肠弯曲菌分别占58.6%、4.3%和37.1%;人源菌和鸡源菌均以空肠弯曲菌生物1型为主,猪源菌主要为结肠弯曲菌。弯曲菌质粒检出率为18.9%,检出的质粒株中,大多含2~3个质粒带,分子量﹤1.4~58×106道尔顿;结肠弯曲菌的质粒检出率(34.21%)明显高于空肠弯曲菌(10.29%)。两个病家的调查结果说明:生物分型和质粒分析在追踪弯曲菌感染的传播中具有一定价值,可以作为弯曲菌感染流行病学调查的有用工具。 |
| English Abstract: |
| Biotyping and plasmid analysis of Campylobacter jejuni/coli from the Chengdu district were made and the methods which combined with the Penner serotyping scheme were applied to family studies of the patients with enteritis of Campylobacter jejuni/coli positive. In a total of 116 isolates, C.jejuni biotype 1, biotype 2 and C.coli comprised 58.6%, 4.3% and 37.1% respectively. Most of Campylobacter jejuni/coli from humans and chickens were C.jejuni biotype 1, whereas C.coli was the predominant organism in pigs. Of the 106 strains, plasmid occurrence rate was 18.9%. Most strains which carried plasmids had between 2 and 3 of them. Plasmids ranged in size from<1.4 to 58 megadaltons. The incidence of plasmids observed in C.coli strains (34.21%) was significantly greater than that observed in C.jejuni strains (10.29%). The study showed that the biotyping and plasmid analysis were valuable in tracing transmission of Campylobacter infection and might be useful tools in epidemiological survey of Campylobacter infection. |
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