鼠疫耶尔森菌rRNA基因识别特征研究

张慧娟; 魏建春; 张恩民; 申小娜; 梁莹; 朱晓宇; 徐冬蕾; 孙丽娜; 张建华; 俞东征; 海荣

Abstract

张慧娟,魏建春,张恩民,申小娜,梁莹,朱晓宇,徐冬蕾,孙丽娜,张建华,俞东征,海荣.鼠疫耶尔森菌rRNA基因识别特征研究[J].Chinese journal of Epidemiology,2011,32(4):366-369

鼠疫耶尔森菌rRNA基因识别特征研究

Study on the identiffcation characteristies of dRNA genes On Yersinia pestis

Received:December 24, 2010

DOI：

KeyWord: 鼠疫耶尔森菌 rRNA基因识别特征

English Key Word: rsiniapestis rRNAgenes Identification characteristics

FundProject:国家“十五”科技攻关项目(2004BA718807)；国家科技部重大专项(2008ZXl0004-008)；卫生部专项经费资助项目(200802016)

Author Name	Affiliation	E-mail
Zhang Huijuan	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wei Jianchun	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhang Enmin	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Shen Xiaona	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Liang Ying	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhu Xiaoyu	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xu Donglei	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Sun Lina	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhang Jianhua	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Yu Dongzheng	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China	yudongzheng@icdc．Cn
Hai Rong	State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China	haimng@icdc.cn

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Abstract:

目的分析鼠疫耶尔森菌(鼠疫菌)的rRNA基因识别特征。方法通过比较基因组学方法，利用Clustal W软件，对目前已完成全基因组测序的9株鼠疫菌的rRNA基因序列进行分析，分别比对rRNA基因簇两侧的2000bp序列、基因簇内16S、23S、5S rRNA及16S～23S基因间隔区序列，以确定鼠疫菌rRNA基凶的识别特征。结果菌株D182038、D106004、Z176003和C092分别有6个rRNA基因簇拷贝(6拷贝菌株)，菌株91001、KIM、Nepal516、Antiqua和PestoidesF分别有7个rRNA基因簇拷贝(7拷贝菌株)。按照两侧2000bp序列不同，可将鼠疫菌rRNA基因簇分成13种类型，并可将6拷贝与7拷贝菌株进行区分；16S一23S rRNA基因间隔区含有4种不同种类和数量的tRNA基因，可将6拷贝菌株和7拷贝菌株分别进行区分；23S rRNA基因在不同菌株间及不同rRNA拷贝间的碱基突变数方差分析显示，各菌株间F=0．548，P=0．815>0．05；各拷贝间F=5．228，P<0．01。结论鼠疫菌rRNA基因簇两侧序列、间隔区tRNA基因和23S rRNA基因可作为识别不同菌株和不同rRNA基因簇拷贝的指标，将鼠疫菌不同菌株进行分类。

English Abstract:

Objective To study the identification characteristics of rRNA genes on Yeninia (K)pestis．Methods By means of comparative genomics，we compared the rRNA genome sequences of nine completely sequenced strains of M pestis isolated from China and other countries by Clustal W software．We alSO compared the 2000 bp sequence adjacent to the rRNA genes．rRNA genes and 16S一23S rRNA spacer region respectively to determine the identification features of rRNA genes for y．pestis．Results There were 6 rRNA gene clusters in the strains of D182038，D106004， Z176003 and C092 respectively(6 copies strain)．There were 7 rRNA gene clusters in the strains of 91001，ⅪM，Nepal516，Antiqua and Pestoides F(7 copies strain)．According to the 2000 bp sequence，13 types ofrRNA gene clusters could classify the strains between the 6 copies and 7 copies．There were 4 types of tRNA gene among the 16S-23S rRNA spacer region that could classify the trains among the 6 copies and 7 copies strains respectively．ne number of point mutation among the 23S rRNA gene was statistically different in some copies under ANOVA analysis(F=O．548，P=0．815>0．05 among the strains and F=5．228．PConclusion The 2000 bpsequence甜jacent to the rRNA genes，tRNA gene and 23S rRNA gene sequence could serve as the identification sign of rRNA genes for classifing the strains ofY pestis．

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